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POLB ELISA 试剂盒

The 人 POLB ELISA Kit (ABIN7830558) is a Colorimetric ELISA Kit designed to quantify 人 POLB.
产品编号 ABIN7830558
发货至: 中国
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Quick Overview for POLB ELISA 试剂盒 (ABIN7830558)

抗原

See all POLB ELISA试剂盒
POLB (Polymerase (DNA Directed), beta (POLB))

适用

  • 1
  • 1
  • 1

检测方法

Colorimetric

实验类型

Cell ELISA

应用范围

ELISA

样品类型

Cell Samples, Suspension Cell Culture
  • 原理

    POLB (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of POLB expression in cultured cells.

    Analytical Method

    Qualitative

    交叉反应

    小鼠, 大鼠
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    板类型

    Uncoated

    实验流程

    1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
    2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
    3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
    4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

    限制

    仅限研究用
  • 储存条件

    4 °C

    储存方法

    Store the kit at 4°C.
  • 抗原 See all POLB ELISA试剂盒

    POLB (Polymerase (DNA Directed), beta (POLB))

    别名

    POLB

    背景

    Synonyms: MGC125976

    Gene Description: polymerase (DNA directed), beta

    基因ID

    5423

    UniProt

    P06746

    途径

    DNA Damage Repair
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