Cyclin A1 ELISA 试剂盒
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Quick Overview for Cyclin A1 ELISA 试剂盒 (ABIN7830536)
抗原
See all Cyclin A1 (CCNA1) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- CCNA1/CCNA2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CCNA1/CCNA2 expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠, 大鼠
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Cyclin A1 (CCNA1) ELISA Kit
CCNA1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
Cyclin A1 (CCNA1) ELISA KitCCNA1 适用: 大鼠 Colorimetric Sandwich ELISA 0.156-10 ng/mL Plasma, Serum
Cyclin A1 (CCNA1) ELISA KitCCNA1 适用: 小鼠 Colorimetric Sandwich ELISA 78.125-5000 pg/mL Plasma, Serum
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- Cyclin A1 (CCNA1)
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别名
- CCNA1
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背景
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Synonyms: -
Gene Description: cyclin A1
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基因ID
- 8900
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UniProt
- P78396
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途径
- Apoptosis, Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA
抗原 See all Cyclin A1 (CCNA1) ELISA试剂盒
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