CTNNB1 ELISA 试剂盒
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Quick Overview for CTNNB1 ELISA 试剂盒 (ABIN7830475)
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See all CTNNB1 ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- CTNNB1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CTNNB1 expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠, 大鼠
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Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) ELISA Kit
CTNNB1 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) ELISA KitCTNNB1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) ELISA KitCTNNB1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) ELISA KitCTNNB1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))
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别名
- CTNNB1
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背景
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Synonyms: CTNNB|DKFZp686D02253|FLJ25606|FLJ37923
Gene Description: catenin (cadherin-associated protein), beta 1, 88 kDa
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基因ID
- 1499
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UniProt
- P35222
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途径
- WNT signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2
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