PRAS40 ELISA 试剂盒
The 人 PRAS40 ELISA Kit (ABIN7830405) is a Colorimetric ELISA Kit designed to quantify 人 PRAS40.
产品编号 ABIN7830405
发货至:
中国
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Contact Info
Our Local Distributor
中国
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北京 101111
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Quick Overview for PRAS40 ELISA 试剂盒 (ABIN7830405)
抗原
See all PRAS40 (AKT1S1) ELISA试剂盒
PRAS40 (AKT1S1)
(AKT1 Substrate 1 (Proline-Rich) (AKT1S1))
适用
人
检测方法
Colorimetric
实验类型
Cell ELISA
应用范围
ELISA
样品类型
Cell Samples, Suspension Cell Culture
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原理
- AKT1S1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of AKT1S1 expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠, 大鼠
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))
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别名
- AKT1S1
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背景
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Synonyms: Lobe|MGC2865|PRAS40
Gene Description: AKT1 substrate 1 (proline-rich)
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基因ID
- 84335
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UniProt
- Q96B36
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途径
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Cell Size, Autophagy, BCR Signaling, Warburg Effect
抗原 See all PRAS40 (AKT1S1) ELISA试剂盒
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