14-3-3 zeta ELISA 试剂盒
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Quick Overview for 14-3-3 zeta ELISA 试剂盒 (ABIN7830383)
抗原
See all 14-3-3 zeta (YWHAZ) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- YWHAZ (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of YWHAZ expression in cultured cells.
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Analytical Method
- Qualitative
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交叉反应
- 小鼠, 大鼠
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14-3-3 zeta (YWHAZ) ELISA Kit
YWHAZ 适用: 人 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
14-3-3 zeta (YWHAZ) ELISA KitYWHAZ 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
14-3-3 zeta (YWHAZ) ELISA KitYWHAZ 适用: 人, 大鼠, 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Uncoated
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实验流程
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Store the kit at 4°C.
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- 14-3-3 zeta (YWHAZ)
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别名
- YWHAZ
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背景
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Synonyms: KCIP-1|MGC111427|MGC126532|MGC138156
Gene Description: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
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基因ID
- 7534
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UniProt
- P63104
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途径
- Apoptosis, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location
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