NOXA ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for NOXA ELISA 试剂盒 (ABIN7672031)
抗原
See all NOXA (PMAIP1) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
质量等级
-
-
最低检测浓度
- 0.313 ng/mL
-
原理
- Rat PMAIP1 (phorbol-12myristate-13-acetate-induced protein 1) QuickTest ELISA Kit
-
Analytical Method
- Quantitative
-
特异性
- Specifically binds with PMAIP1 , no obvious cross reaction with other analogues.
-
灵敏度
- 0.188 ng/mL
-
组件
-
- ELISA Microplate(Dismountable)
- Lyophilized Standard
- Cap/Det Ab (Ready to use
- blue)
- TMB Substrate
- Sample Dilution Buffer
- Stop Solution
- Plate Sealer
- Product Description
-
试剂未包括
-
- Microplate reader (wavelength: 450nm)
- 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5 mL pipettor
- Precision single (0.5-10μL
- 5-50μL
- 20-200μL
- 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
-
-
Phorbol-12-Myristate-13-Acetate-Induced Protein 1 (PMAIP1) ELISA Kit
PMAIP1 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Phorbol-12-Myristate-13-Acetate-Induced Protein 1 (PMAIP1) ELISA KitPMAIP1 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Phorbol-12-Myristate-13-Acetate-Induced Protein 1 (PMAIP1) ELISA KitPMAIP1 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Phorbol-12-Myristate-13-Acetate-Induced Protein 1 (PMAIP1) ELISA KitPMAIP1 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
说明
-
Sandwich ELISA, Double Antibody
-
样本量
- 50 μL
-
实验时间
- 2 h
-
板类型
- Pre-coated
-
实验流程
- Step 1: Take out the required plate wells, add 50 µL Cap/Det Ab into each well, then add 50 µL Standard or Sample into individual well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Gently tap the plate for 10s to ensure thorough mixing then static incubate for 60 minutes at 37 °C. Washing: Wash the plate twice without immersion. Step 2: Add 100 µL HRP-Streptavidin (orange) into each well, seal the plate and static incubate for 30 minutes at 37 °C. Washing: Wash the plate five times without immersion. Step 3: Add 90 µL TMB substrate solution, seal the plate and static incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 4: Add 50 µL stop solution. Read at 450nm immediately and calculate.
-
实验流程
-
- Take out the required strips, add 50 µL Cap/Det Ab into each well, then add 50 µL Standard or Sample into relevant well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Gently tap the plate for 10s to ensure complete mixing then statically incubate for 60 minutes at 37 °C.
- Wash the plate twice without immersion.
- Add 100 µL HRP-Streptavidin (orange) into each well, seal the plate and statically incubate for 30 minutes at 37 °C.
- Wash the plate five times without immersion.
- Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C (Accurate TMB visualization control is required.).
- Add 50 µL stop solution. Read at 450nm immediately and calculate.
-
限制
- 仅限研究用
-
-
-
注意事项
- Avoid direct light exposure to antibody solutions
-
储存条件
- 4 °C
-
储存方法
- Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant
-
有效期
- 12 months
-
-
- NOXA (PMAIP1) (Phorbol-12-Myristate-13-Acetate-Induced Protein 1 (PMAIP1))
-
别名
- PMAIP1
-
背景
- Phorbol-12-myristate-13-acetate-induced protein 1, PMA-induced protein 1, Immediate-early-response protein APR, Protein Noxa, PMAIP1, NOXA
-
UniProt
- Q5U777
-
途径
- p53 Pathway, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus
抗原 See all NOXA (PMAIP1) ELISA试剂盒
-
