IL-18 ELISA 试剂盒
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北京 101111
Quick Overview for IL-18 ELISA 试剂盒 (ABIN7671688)
抗原
See all IL-18 (IL18) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
质量等级
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最低检测浓度
- 15.625 pg/mL
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原理
- Human IL-18 (Interleukin 18) QuickTest ELISA Kit
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Analytical Method
- Quantitative
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特异性
- Specifically binds with IL-18 , no obvious cross reaction with other analogues. Recommended Sample Dilution Ratio The following table shows the recommended dilution ratios for this kit for a limited number of samples for your reference only. (When the sample content is very low, the original solution can be added without dilution, but it is necessary to ensure that the pH is between 6.8 and 8.0, and it does not contain more than 10 % organic solvents or high-concentration protein denaturants.) 1. Common sample validation: Sample Type Recommended Dilution Ratio Content Healthy serum (n=20) undiluted 17-355pg/mL Healthy plasma (EDTA, Citrate , heparin) (n=22) undiluted 18-386pg/mL THP-1 cells were cultured in RPMI 1640 supplemented with 80 nM TPA, 10 %FBS, 100 units/mL penicillin, and 100 μg/mL streptomycin sulfate, incubated overnight. The cell culture supernatants were assayed for levels of human IL-18. undiluted ND THP-1 cells were cultured in RPMI 1640 supplemented with 80 nM TPA, 10 %FBS, 100 units/mL penicillin, and 100 μg/mL streptomycin sulfate, incubated overnight. Cells were then stimulated with 100 ng/mL LPS for 12 hours, and the cell culture supernatants were assayed for levels of human IL-18. undiluted 496pg/mL Hela cells were cultured in DMEM supplemented with 10 %FBS, 100 units/mL penicillin, and 100 μg/mL streptomycin sulfate for 12 hours. The cell culture supernatants were assayed for levels of human IL-18. undiluted 267pg/mL 2. KO sample validation (Detect IL-18 KO Hela cells): Sample Type Dilution Ratio Content Wild Hela cells were cultured in DMEM supplemented with 10 %FBS, 100 units/mL penicillin, and 100 μg/mL streptomycin sulfate for 12 hours. The cell culture supernatants were assayed for levels of human IL-18. undiluted 318pg/mL KO Hela cells were cultured in DMEM supplemented with 10 %FBS, 100 units/mL penicillin, and 100 μg/mL streptomycin sulfate for 12 hours. The cell culture supernatants were assayed for levels of human IL-18. undiluted ND Note:ND is lower than the sensitivity of the kit and was not detected 3. Verified recombinant protein: (It is a normal phenomenon that some proteins have weak detection signals or cannot be detected at all due to differences in tags, sequences or protein activities) A.E. coli-derived Human IL-18 protein Tyr37-Asp193
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灵敏度
- 9.375 pg/mL
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组件
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- ELISA Microplate(Dismountable)
- Lyophilized Standard
- Cap/Det Ab (Ready to use
- blue)
- TMB Substrate
- Sample Dilution Buffer
- Stop Solution
- Plate Sealer
- Product Description
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试剂未包括
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- Microplate reader (wavelength: 450nm)
- 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5 mL pipettor
- Precision single (0.5-10μL
- 5-50μL
- 20-200μL
- 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
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Interleukin 18 (IL18) ELISA Kit
IL18 适用: 人 Colorimetric Sandwich ELISA 31.25-2000 pg/mL Plasma, Serum, Tissue Homogenate, Urine
Interleukin 18 (IL18) ELISA KitIL18 适用: Cow Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 18 (IL18) ELISA KitIL18 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 18 (IL18) ELISA KitIL18 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 18 (IL18) ELISA KitIL18 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate, Urine
Interleukin 18 (IL18) ELISA KitIL18 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 18 (IL18) ELISA KitIL18 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate, Urine
Interleukin 18 (IL18) ELISA KitIL18 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 18 (IL18) ELISA KitIL18 适用: Pig Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 18 (IL18) ELISA KitIL18 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Sandwich ELISA, Double Antibody
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样本量
- 50 μL
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实验时间
- 2 h
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板类型
- Pre-coated
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实验流程
- Step 1: Take out the required plate wells, add 50 µL Cap/Det Ab into each well, then add 50 µL Standard or Sample into individual well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Gently tap the plate for 10s to ensure thorough mixing then static incubate for 60 minutes at 37 °C. Washing: Wash the plate twice without immersion. Step 2: Add 100 µL HRP-Streptavidin (orange) into each well, seal the plate and static incubate for 30 minutes at 37 °C. Washing: Wash the plate five times without immersion. Step 3: Add 90 µL TMB substrate solution, seal the plate and static incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 4: Add 50 µL stop solution. Read at 450nm immediately and calculate.
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实验流程
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- Take out the required strips, add 50 µL Cap/Det Ab into each well, then add 50 µL Standard or Sample into relevant well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Gently tap the plate for 10s to ensure complete mixing then statically incubate for 60 minutes at 37 °C.
- Wash the plate twice without immersion.
- Add 100 µL HRP-Streptavidin (orange) into each well, seal the plate and statically incubate for 30 minutes at 37 °C.
- Wash the plate five times without immersion.
- Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C (Accurate TMB visualization control is required.).
- Add 50 µL stop solution. Read at 450nm immediately and calculate.
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限制
- 仅限研究用
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注意事项
- Avoid direct light exposure to antibody solutions
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储存条件
- 4 °C
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储存方法
- Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant
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有效期
- 12 months
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- IL-18 (IL18) (Interleukin 18 (IL18))
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别名
- IL-18
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背景
- Interleukin-18, IL-18, Iboctadekin, Interferon gamma-inducing factor, IFN-gamma-inducing factor, Interleukin-1 gamma, IL-1 gamma, IL18, IGIF, IL1F4
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UniProt
- Q14116
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途径
- Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
抗原 See all IL-18 (IL18) ELISA试剂盒
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