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Survivin ELISA 试剂盒

BIRC5 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
产品编号 ABIN6951658
发货至: 中国
  • 抗原 See all Survivin (BIRC5) ELISA试剂盒
    Survivin (BIRC5) (Baculoviral IAP Repeat-Containing 5 (BIRC5))
    适用
    • 5
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human Survivin/BIRC5 ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product BIRC5 ELISA Kit
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 有效期
    6 months
  • 抗原 See all Survivin (BIRC5) ELISA试剂盒
    Survivin (BIRC5) (Baculoviral IAP Repeat-Containing 5 (BIRC5))
    别名
    Survivin (BIRC5 (BIRC5 产品)
    别名
    AP14 ELISA Kit, API4 ELISA Kit, EPR-1 ELISA Kit, AAC-11 ELISA Kit, Api4 ELISA Kit, TIAP ELISA Kit, survivin40 ELISA Kit, bir5a ELISA Kit, survivin ELISA Kit, BIR1 ELISA Kit, Su2 ELISA Kit, XBIR1 ELISA Kit, XSurvivin1 ELISA Kit, Xsvv1 ELISA Kit, api4 ELISA Kit, bir1-A ELISA Kit, epr-1 ELISA Kit, svv1 ELISA Kit, xSurvivin ELISA Kit, xsurv ELISA Kit, Su1 ELISA Kit, birc5.2 ELISA Kit, svv2 ELISA Kit, xSurvivin2 ELISA Kit, bir5b ELISA Kit, SIX ELISA Kit, birc5.2-a ELISA Kit, birc5.2-b ELISA Kit, baculoviral IAP repeat containing 5 ELISA Kit, baculoviral IAP repeat-containing 5 ELISA Kit, baculoviral IAP repeat containing 5a ELISA Kit, baculoviral IAP repeat-containing 5, gene 1 L homeolog ELISA Kit, baculoviral IAP repeat containing 5 L homeolog ELISA Kit, baculoviral IAP repeat containing 5b ELISA Kit, baculoviral IAP repeat containing 5 S homeolog ELISA Kit, BIRC5 ELISA Kit, Birc5 ELISA Kit, birc5a ELISA Kit, birc5.1.L ELISA Kit, birc5.2.L ELISA Kit, birc5b ELISA Kit, birc5.2.S ELISA Kit
    基因ID
    332
    UniProt
    O15392
    途径
    Apoptosis, Cell Division Cycle, Nuclear Hormone Receptor Binding
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