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ATG4B ELISA 试剂盒

This anti-ATG4B antibody (ABIN6951529) is a antibody detecting ATG4B in ELISA. Suitable for Human.
产品编号 ABIN6951529
发货至: 中国

Quick Overview for ATG4B ELISA 试剂盒 (ABIN6951529)

抗原

See all ATG4B products
ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))

适用

检测方法

Colorimetric

实验类型

Sandwich ELISA

应用范围

ELISA

样品类型

Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
  • 原理

    Human ATG4B ELISA Kit.

    Analytical Method

    Quantitative

    产品特性

    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data

    组件

    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate

    试剂未包括

    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    板类型

    Pre-coated

    实验流程

    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.

    限制

    仅限研究用
  • 有效期

    6 months
  • 抗原 See all ATG4B products

    ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))

    别名

    ATG4B

    基因ID

    23192

    UniProt

    Q9Y4P1

    途径

    Autophagy
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