ADAM17 ELISA 试剂盒
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- 抗原 See all ADAM17 ELISA试剂盒
- ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Rat ADAM17/TACE ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product ADAM17 ELISA Kit
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ADAM Metallopeptidase Domain 17 (ADAM17) ELISA Kit
ADAM17 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
SensoLyte® 520 TACE (a-Secretase) Activity Assay KitAct, FRET 适用: 人 Fluorometric
ADAM Metallopeptidase Domain 17 (ADAM17) ELISA KitADAM17 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
ADAM Metallopeptidase Domain 17 (ADAM17) ELISA KitADAM17 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
ADAM Metallopeptidase Domain 17 (ADAM17) ELISA KitADAM17 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
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- 抗原 See all ADAM17 ELISA试剂盒
- ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))
- 别名
- ADAM17 (TACE (ADAM17 产品)
- 别名
- ADAM18 ELISA Kit, CD156B ELISA Kit, CSVP ELISA Kit, NISBD ELISA Kit, TACE ELISA Kit, CD156b ELISA Kit, Tace ELISA Kit, adam17 ELISA Kit, si:dkey-81b15.2 ELISA Kit, adam18 ELISA Kit, cd156b ELISA Kit, csvp ELISA Kit, tace ELISA Kit, fc20b01 ELISA Kit, wu:fc20b01 ELISA Kit, zgc:63886 ELISA Kit, ADAM metallopeptidase domain 17 ELISA Kit, a disintegrin and metallopeptidase domain 17 ELISA Kit, ADAM metallopeptidase domain 17b ELISA Kit, ADAM metallopeptidase domain 17 L homeolog ELISA Kit, ADAM metallopeptidase domain 17a ELISA Kit, ADAM17 ELISA Kit, Adam17 ELISA Kit, adam17b ELISA Kit, adam17.L ELISA Kit, adam17a ELISA Kit
- 基因ID
- 57027
- UniProt
- Q9Z1K9
- 途径
- Notch Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus
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