我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

M-CSF/CSF1 ELISA 试剂盒

Name: M-CSF/CSF1 ELISA 试剂盒
SKU: ABIN6951452
Availability: OutOfStock

This 小鼠 M-CSF/CSF1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 M-CSF/CSF1.

产品编号 ABIN6951452

发货至: 中国

Quick Overview for M-CSF/CSF1 ELISA 试剂盒 (ABIN6951452)

抗原

See all M-CSF/CSF1 (CSF1) ELISA试剂盒

M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

适用

小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

1 pg/mL - 350 pg/mL

应用范围

ELISA

样品类型

Cell Lysate, Tissue Lysate

Discover our top product CSF1 ELISA Kit

最低检测浓度

1 pg/mL

原理

Mouse M-CSF ELISA Kit for cell and tissue lysate samples.

Analytical Method

Quantitative

特异性

This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.

产品特性
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
组件
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
试剂未包括
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Colony Stimulating Factor 1 (Macrophage) (CSF1) ELISA Kit
CSF1 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate, Urine

Colony Stimulating Factor 1 (Macrophage) (CSF1) ELISA Kit
CSF1 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Colony Stimulating Factor 1 (Macrophage) (CSF1) ELISA Kit
CSF1 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Colony Stimulating Factor 1 (Macrophage) (CSF1) ELISA Kit
Small Sample CSF1 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate, Urine

Colony Stimulating Factor 1 (Macrophage) (CSF1) ELISA Kit
CSF1 适用: 猴 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum

Colony Stimulating Factor 1 (Macrophage) (CSF1) ELISA Kit
CSF1 适用: 猴 Colorimetric Sandwich ELISA 39.07 pg/mL - 2500 pg/mL Plasma, Serum, Tissue Homogenate

Colony Stimulating Factor 1 (Macrophage) (CSF1) ELISA Kit
CSF1 适用: 人 AA 33-190 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Tissue Homogenate, Urine

Colony Stimulating Factor 1 (Macrophage) (CSF1) ELISA Kit
CSF1 适用: 人 Colorimetric Sandwich ELISA 0.313-5 ng/mL Cell Culture Cells, Milk, Plasma, Serum

Colony Stimulating Factor 1 (Macrophage) (CSF1) ELISA Kit
CSF1 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Serum, Tissue Lysates

Colony Stimulating Factor 1 (Macrophage) (CSF1) ELISA Kit
CSF1 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum, Tissue Lysates, Urine

应用备注

Optimal working dilution should be determined by the investigator.

板类型

Pre-coated

实验流程
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.
限制

仅限研究用
储存方法

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

有效期

6 months
抗原 See all M-CSF/CSF1 (CSF1) ELISA试剂盒

M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

别名

M-CSF

基因ID

12977

UniProt

P07141

途径

RTK signaling