WISP1 ELISA 试剂盒
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- 抗原 See all WISP1 ELISA试剂盒
- WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Mouse WISP-1 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product WISP1 ELISA Kit
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WNT1 Inducible Signaling Pathway Protein 1 (WISP1) ELISA Kit
WISP1 适用: 人 Colorimetric Sandwich ELISA 78.13 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
WNT1 Inducible Signaling Pathway Protein 1 (WISP1) ELISA KitWISP1 适用: 小鼠 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Plasma, Serum, Tissue Homogenate
WNT1 Inducible Signaling Pathway Protein 1 (WISP1) ELISA KitWISP1 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
WNT1 Inducible Signaling Pathway Protein 1 (WISP1) ELISA KitWISP1 适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
WNT1 Inducible Signaling Pathway Protein 1 (WISP1) ELISA KitWISP1 适用: 小鼠 AA 23-367 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
WNT1 Inducible Signaling Pathway Protein 1 (WISP1) ELISA KitWISP1 适用: 人 AA 23-367 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 有效期
- 6 months
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- 抗原 See all WISP1 ELISA试剂盒
- WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))
- 别名
- WISP-1 (WISP1 产品)
- 别名
- CCN4 ELISA Kit, WISP1c ELISA Kit, WISP1i ELISA Kit, WISP1tc ELISA Kit, WISP-1 ELISA Kit, WISP1 ELISA Kit, si:ch211-38f10.2 ELISA Kit, AW146261 ELISA Kit, Elm1 ELISA Kit, zgc:172276 ELISA Kit, WNT1 inducible signaling pathway protein 1 ELISA Kit, WNT1 inducible signaling pathway protein 1a ELISA Kit, WNT1-inducible-signaling pathway protein 1-like ELISA Kit, WNT1 inducible signaling pathway protein 1b ELISA Kit, WISP1 ELISA Kit, wisp1a ELISA Kit, Wisp1 ELISA Kit, LOC100627044 ELISA Kit, wisp1b ELISA Kit
- 基因ID
- 22402
- UniProt
- O54775
- 途径
- WNT signaling, Growth Factor Binding
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