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ATOX1 ELISA 试剂盒
This 人 ATOX1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 ATOX1 in Cell Culture Supernatant, Cell Samples, Plasma, Serum 和 Tissue Lysate.
Quick Overview for ATOX1 ELISA 试剂盒 (ABIN6951064)
抗原
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ATOX1
(ATX1 Antioxidant Protein 1 Homolog (ATOX1))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
样品类型
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
ATOX1ELISA Kit详情
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原理
Human ATOX1 ELISA Kit.
Analytical Method
Quantitative
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
使用细节
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应用备注
Optimal working dilution should be determined by the investigator.
板类型
Pre-coated
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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有效期
6 months
ATOX1目标详情
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ATOX1
(ATX1 Antioxidant Protein 1 Homolog (ATOX1))
别名
ATOX1
基因ID
475
UniProt
O00244
途径
Transition Metal Ion Homeostasis
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