抗原 See all CCL2 IQ-ELISA Kits

CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

适用 12 9 7 4 3 3 2 2 2 2 2 2 1 1

人

检测方法

qPCR

实验类型

DNA-Binding ELISA

检测范围

0.032 pg/mL - 500 pg/mL

最低检测浓度

0.032 pg/mL

应用范围

ELISA

原理

Human Immunoquantitative (PCR-Based) MCP-1 ELISA Kit for cell culture supernatants, plasma, and serum samples.

样品类型

Cell Culture Supernatant, Plasma, Serum

Analytical Method

Quantitative

灵敏度

0.032 pg/mL

产品特性

The Immuno Quantitative Enzyme Linked ImumunoSorbent Assay (IQELISA™) is an innovative new assay that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of lower sample volumes while also providing more sensitivity. The Human MCP-1 IQELISA™ Kit is a modified ELISA assay with high sensitivity qPCR readout for the quantitative measurement of Human MCP-1 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for Human MCP-1 coated on a 96-well PCR plate. Standards and samples are pipetted into the wells and MCP-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and a detection affinity molecule is added to the plates. After washing away unbound detection affinity molecule, primers and a PCR master mix are added to the wells and data is collected using qPCR. Ct values obtained from the qPCR are then used to calculate the amount of antigen contained in each sample, where lower Ct values indicate a higher concentration of antigen.

组件

MCP-1 Microplate (Item A): 96 well PCR plate coated with anti-Human MCP-1
Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
Standards (Item C): 2 vials of recombinant Human MCP-1
Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
Detection Affinity Reagent for MCP-1 (Item F): 2 vials of a 4x concentrated solution of anti-Human MCP-1 affinity reagent
IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
Primer Solution (Item I): 1.7 ml vial
PCR Master Mix (Item J): 1.2 ml vial
PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer

试剂未包括

Real-time PCR instrument, Bio-Rad recommended
Precision pipettes to deliver 2 μL to 1 mL volumes
Adjustable 1-25 mL pipettes for reagent preparation
100 mL and 1 liter graduated cylinders
Absorbent paper
Distilled or deionized water
Log-log graph paper or computer and software for data analysis
Tubes to prepare standard or sample dilutions
Heating block or water bath capable of 80^°C

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