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KIT ELISA 试剂盒
KIT
适用: 小鼠
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
KITELISA Kit详情
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KIT
(Mast/stem Cell Growth Factor Receptor (KIT))
适用
All reactivities for KIT ELISA试剂盒
小鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
原理
Mouse CD117/c-kit ELISA Kit.
样品类型
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
特异性
This ELISA antibody pair recognizes Mouse CD117.
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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使用细节
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应用备注
Optimal working dilution should be determined by the investigator.
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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储存方法
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
有效期
6 months
KIT目标详情
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KIT
(Mast/stem Cell Growth Factor Receptor (KIT))
别名
CD117 (c-kit (KIT 产品 )
别名
C-Kit ELISA Kit, CD117 ELISA Kit, PBT ELISA Kit, SCFR ELISA Kit, Bs ELISA Kit, Fdc ELISA Kit, Gsfsco1 ELISA Kit, Gsfsco5 ELISA Kit, Gsfsow3 ELISA Kit, SCO1 ELISA Kit, SCO5 ELISA Kit, SOW3 ELISA Kit, Ssm ELISA Kit, Tr-kit ELISA Kit, W ELISA Kit, c-KIT ELISA Kit, c-kit ELISA Kit, MGF ELISA Kit, KIT ELISA Kit, C-KIT ELISA Kit, kit ELISA Kit, receptor ELISA Kit, sparse ELISA Kit, Xkl-1 ELISA Kit, kit-a ELISA Kit, kit-b ELISA Kit, kl1-A ELISA Kit, krk1 ELISA Kit, pbt ELISA Kit, scfr ELISA Kit, KIT proto-oncogene receptor tyrosine kinase ELISA Kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog a ELISA Kit, KIT proto-oncogene receptor tyrosine kinase S homeolog ELISA Kit, KIT ELISA Kit, Kit ELISA Kit, kita ELISA Kit, kit ELISA Kit, kit.S ELISA Kit
UniProt
P05532
途径
RTK signaling , Fc-epsilon Receptor Signaling Pathway , EGFR Signaling Pathway , Neurotrophin Signaling Pathway , Sensory Perception of Sound , Stem Cell Maintenance , Production of Molecular Mediator of Immune Response , Regulation of long-term Neuronal Synaptic Plasticity
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