EPH Receptor B3 ELISA 试剂盒
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- 抗原 See all EPH Receptor B3 (EPHB3) ELISA试剂盒
- EPH Receptor B3 (EPHB3)
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Mouse EphB3 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Mouse EphB3.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product EPHB3 ELISA Kit
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EPH Receptor B3 (EPHB3) ELISA Kit
EPHB3 适用: 小鼠, 人 pTyr Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
EPH Receptor B3 (EPHB3) ELISA KitEPHB3 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
EPH Receptor B3 (EPHB3) ELISA KitEPHB3 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all EPH Receptor B3 (EPHB3) ELISA试剂盒
- EPH Receptor B3 (EPHB3)
- 别名
- EphB3 (EPHB3 产品)
- 别名
- tck ELISA Kit, MGC83457 ELISA Kit, EPHB3 ELISA Kit, ETK2 ELISA Kit, HEK2 ELISA Kit, TYRO6 ELISA Kit, AW456895 ELISA Kit, Cek10 ELISA Kit, Etk2 ELISA Kit, MDK5 ELISA Kit, Sek4 ELISA Kit, Tyro6 ELISA Kit, CEK10 ELISA Kit, EK10 ELISA Kit, ek3 ELISA Kit, ephb3 ELISA Kit, fc62d03 ELISA Kit, rtk3 ELISA Kit, wu:fc62d03 ELISA Kit, zek3 ELISA Kit, EPH receptor B3 ELISA Kit, EPH receptor B3 L homeolog ELISA Kit, Eph receptor B3 ELISA Kit, eph receptor B3a ELISA Kit, Eph receptor tyrosine kinase ELISA Kit, EPHB3 ELISA Kit, ephb3.L ELISA Kit, ephb3 ELISA Kit, Ephb3 ELISA Kit, ephb3a ELISA Kit
- UniProt
- P54754
- 途径
- RTK signaling
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