FZD2 ELISA 试剂盒
FZD2
适用: 小鼠
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all FZD2 products
- FZD2 (Frizzled Family Receptor 2 (FZD2))
- 适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Mouse Frizzled-2 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Mouse Frizzled-2.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all FZD2 products
- FZD2 (Frizzled Family Receptor 2 (FZD2))
- 别名
- Frizzled-2 (FZD2 产品)
- 别名
- BG01711 ELISA Kit, CG9739 ELISA Kit, D-Fz2 ELISA Kit, D-fz2 ELISA Kit, DFZ2 ELISA Kit, DFz-2 ELISA Kit, DFz2 ELISA Kit, Dfrz2 ELISA Kit, Dfz2 ELISA Kit, Dfz[[2]] ELISA Kit, Dm Fz2 ELISA Kit, Dmel\\CG9739 ELISA Kit, FZ2 ELISA Kit, Fz2 ELISA Kit, dFz2 ELISA Kit, dfz(2) ELISA Kit, dfz2 ELISA Kit, dfz2r ELISA Kit, frz2 ELISA Kit, fz-2 ELISA Kit, fz8 ELISA Kit, zfz2 ELISA Kit, zg08 ELISA Kit, cb383 ELISA Kit, wu:fb70g09 ELISA Kit, FZD2 ELISA Kit, fzE2 ELISA Kit, hFz2 ELISA Kit, Fz-10 ELISA Kit, Fzd10 ELISA Kit, cFz-2 ELISA Kit, frizzled2 ELISA Kit, fz2 ELISA Kit, xfz2 ELISA Kit, AL033370 ELISA Kit, AW456835 ELISA Kit, Fz10 ELISA Kit, Mfz10 ELISA Kit, Mfz10a ELISA Kit, frizzled 2 ELISA Kit, frizzled class receptor 2 ELISA Kit, frizzled class receptor 2 S homeolog ELISA Kit, fz2 ELISA Kit, fzd2 ELISA Kit, FZD2 ELISA Kit, Fzd2 ELISA Kit, fzd2.S ELISA Kit
- UniProt
- Q9JIP6
- 途径
- WNT signaling
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