电话:: +86 (0512) 65829739
传真:: +86 (010) 6788 5057
电子邮件:: orders@antibodies-online.cn

我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

ABL1 ELISA 试剂盒

This 人 ABL1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 ABL1.

Jurkat cells were treated with Pervanadate. (ABL1 ELISA 试剂盒)

Jurkat cells were treated with Pervanadate. (ABL1 ELISA 试剂盒)

Jurkat cells were treated with Pervanadate. (ABL1 ELISA 试剂盒)

3 images

产品编号 ABIN6730568

发货至: 中国

Quick Overview for ABL1 ELISA 试剂盒 (ABIN6730568)

抗原

See all ABL1 ELISA试剂盒

ABL1 (C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1))

抗原表位

pTyr245

适用

人

检测方法

Colorimetric

实验类型

Sandwich ELISA

应用范围

ELISA

样品类型

Cell Samples, Tissue Lysate

Discover our best selling ABL1 ELISA Kit

Discover our top product ABL1 ELISA Kit

原理

Human Phospho-c-Abl (Tyr245) and Total c-Abl ELISA Kit. This assay semi-quantitatively measures c-Abl phosphorylated at Tyrosine-245 as well as total c-Abl in cell lysate samples.

Analytical Method

Semi-Quantitative

特异性

This ELISA kit recognizes Human c-Abl phosphorylated at site Tyrosine-245 as well as total c-Abl.

产品特性
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
组件
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
试剂未包括
- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1) ELISA Kit
ABL1 适用: 人 pTyr245 Colorimetric Sandwich ELISA Cell Samples, Tissue Lysate

C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1) ELISA Kit
ABL1 适用: 人 Colorimetric Sandwich ELISA 25 pg/mL - 1600 pg/mL Plasma, Serum, Tissue Homogenate

C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1) FLISA Kit
ABL1 适用: 人, 小鼠, 大鼠 pTyr245 Fluorometric Cell ELISA Cell Culture Cells

C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1) ELISA Kit
ABL1 适用: 人, 小鼠, 大鼠 pTyr204 Colorimetric Cell ELISA Cell Culture Cells

C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1) ELISA Kit
ABL1 适用: 人, 小鼠, 大鼠 pTyr245 Colorimetric Cell ELISA Cell Culture Cells

C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1) ELISA Kit
ABL1 适用: 人, 小鼠, 大鼠 pThr735 Colorimetric Cell ELISA Cell Culture Cells

C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1) ELISA Kit
ABL1 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

应用备注

Optimal working dilution should be determined by the investigator.

实验流程
1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.
限制

仅限研究用
储存条件

-20 °C

储存方法

Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

有效期

6 months
抗原 See all ABL1 ELISA试剂盒

ABL1 (C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1))

别名

c-Abl

基因ID

25

UniProt

P00519

途径

Apoptosis, Regulation of Muscle Cell Differentiation, Platelet-derived growth Factor Receptor Signaling, Lipid Metabolism

You are here: