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UBE2M ELISA 试剂盒

ube2m 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
产品编号 ABIN6730521
发货至: 中国
  • 抗原 See all UBE2M (ube2m) products
    UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))
    适用
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human UBE2M ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes Human UBE2M.
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all UBE2M (ube2m) products
    UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))
    别名
    UBE2M (ube2m 产品)
    别名
    DDBDRAFT_0204633 ELISA Kit, DDBDRAFT_0238042 ELISA Kit, DDB_0204633 ELISA Kit, DDB_0238042 ELISA Kit, UBC-RS2 ELISA Kit, UBC12 ELISA Kit, hUbc12 ELISA Kit, 2510040H03Rik ELISA Kit, Ubc-rs2 ELISA Kit, ubiquitin conjugating enzyme E2M ELISA Kit, ubiquitin-conjugating enzyme E2M ELISA Kit, ubiquitin conjugating enzyme E2 M ELISA Kit, NEDD8-conjugating enzyme ELISA Kit, ubiquitin conjugating enzyme E2M S homeolog ELISA Kit, ube2m ELISA Kit, LOC692934 ELISA Kit, UBE2M ELISA Kit, Ube2m ELISA Kit, ube2m.S ELISA Kit
    UniProt
    P61081
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