CD200 ELISA 试剂盒
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北京 101111
Quick Overview for CD200 ELISA 试剂盒 (ABIN6730437)
抗原
See all CD200 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 28.3 pg/mL
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原理
- Mouse CD200 ELISA Kit for cell culture supernatants, plasma, and serum samples.
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Analytical Method
- Quantitative
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特异性
- This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse 2B4, 4-1BB Ligand, Activin RIB, Ameloblastin, ANGPTL7, B7- 2, B7-H2, B7-H3, B7-H4, BAFF, C1q R1, Cathepsin H, CD117, CD157, CD229, CD28, CD300b, CD39, CD44, CD45, CD69, CD99-L2, CHL-1, CHRDL2, COCO, CRACC, CXADR, DcTRAIL R1, Dectin-2, DNAM-1, DNER, Endoglycan, EphA8, EphB2, EphB4, EphB6, Ephrin-A2, Ephrin-A4, FGF-21.
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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CD200 (CD200) ELISA Kit
CD200 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
CD200 (CD200) ELISA KitCD200 适用: 大鼠 Colorimetric Sandwich ELISA 47 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
CD200 (CD200) ELISA KitCD200 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum, Tissue Homogenate
CD200 (CD200) ELISA KitCD200 适用: 人 AA 31-232 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
CD200 (CD200) ELISA KitCD200 适用: 小鼠 AA 31-232 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
CD200 (CD200) ELISA KitCD200 适用: 人 Colorimetric Sandwich ELISA 12.5 pg/mL - 800 pg/mL Plasma, Serum
CD200 (CD200) ELISA KitCD200 适用: 人 Colorimetric Sandwich ELISA 12.5 pg/mL - 800 pg/mL Cell Culture Supernatant, Plasma, Serum
CD200 (CD200) ELISA KitCD200 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
- Suggested Plasma Dilution: 6 fold
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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有效期
- 6 months
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- CD200
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别名
- CD200
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背景
- Gene Symbols: OX-2 membrane glycoprotein (MRC OX-2 antigen) (CD antigen CD200)
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UniProt
- O54901
抗原 See all CD200 ELISA试剂盒
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