CD200 ELISA 试剂盒
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- 抗原 See all CD200 ELISA试剂盒
- CD200
- 抗原表位
- AA 31-232
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 62.5-4000 pg/mL
- 最低检测浓度
- 62.5 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CD200
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: Q31-G232 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: Q31-G232 - Featured
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- Discover our top product CD200 ELISA Kit
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CD200 (CD200) ELISA Kit
CD200 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
CD200 (CD200) ELISA KitCD200 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum, Tissue Homogenate
CD200 (CD200) ELISA KitCD200 适用: 大鼠 Colorimetric Sandwich ELISA 47 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
CD200 (CD200) ELISA KitCD200 适用: 人 AA 31-232 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
CD200 (CD200) ELISA KitCD200 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
- 板类型
- Pre-coated
- 实验流程
- mouse CD200 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD200 has been precoated onto 96-well plates. Standards(NSO, Q31-G232) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD200 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD200 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse CD200 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CD200 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 341, Standard deviation: 14.66, CV(%): 4.3
- Sample 2: n=16, Mean(pg/ml): 762, Standard deviation: 43.43, CV(%): 5.7
- Sample 3: n=16, Mean(pg/ml): 1104, Standard deviation: 68.45, CV(%): 6.2,
- Sample 1: n=24, Mean(pg/ml): 375, Standard deviation: 23.25, CV(%): 6.2
- Sample 2: n=24, Mean(pg/ml): 790, Standard deviation: 56.09, CV(%): 7.1
- Sample 3: n=24, Mean(pg/ml): 1263, Standard deviation: 85.88, CV(%): 6.8
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all CD200 ELISA试剂盒
- CD200
- 别名
- CD200 (CD200 产品)
- 别名
- MOX1 ELISA Kit, MOX2 ELISA Kit, MRC ELISA Kit, OX-2 ELISA Kit, CD200 ELISA Kit, Cspmo2 ELISA Kit, MRCOX2 ELISA Kit, Mox2 ELISA Kit, DKFZp459C0716 ELISA Kit, OX2 ELISA Kit, CD200 molecule ELISA Kit, Cd200 molecule ELISA Kit, CD200 antigen ELISA Kit, CD200 ELISA Kit, Cd200 ELISA Kit
- 背景
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Protein Function: Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues (By similarity). .
Background: OX-2 membrane glycoprotein, also known as CD200 is a human protein encoded by the CD200 gene. This gene is mapped to 3q13.2. The protein encoded by this gene is a type-1 membrane glycoprotein, which contains two immunoglobulin domains, and thus belongs to the immunoglobulin superfamily. Studies of the related genes in mouse and rat suggest that this gene may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. It is suggested that CD200-CD200R signaling plays a central role in macrophage fusion and osteoclast formation.
Synonyms: OX-2 membrane glycoprotein,MRC OX-2 antigen,CD200,Cd200,Mox2,
Full Gene Name: OX-2 membrane glycoprotein
Cellular Localisation: Cell membrane, Single-pass type I membrane protein. - 基因ID
- 17470
- UniProt
- O54901
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