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PD-L1 ELISA 试剂盒

Name: PD-L1 ELISA 试剂盒
SKU: ABIN6730418
Availability: OutOfStock

This Mouse PD-L1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse PD-L1 in Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate.

产品编号 ABIN6730418

发货至: 中国

Datasheet as PDF

Quick Overview for PD-L1 ELISA 试剂盒 (ABIN6730418)

抗原

See all PD-L1 ELISA试剂盒

PD-L1 (CD274 (PD-L1))

适用

小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

应用范围

ELISA

样品类型

Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

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原理

Mouse PD-L1/B7-H1 ELISA Kit.

Analytical Method

Quantitative

特异性

This ELISA antibody pair recognizes Mouse PD-L1.

产品特性
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
组件
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
试剂未包括
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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应用备注

Optimal working dilution should be determined by the investigator.

实验流程
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.
限制

仅限研究用
储存方法

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

有效期

6 months
抗原 See all PD-L1 ELISA试剂盒

PD-L1 (CD274 (PD-L1))

别名

PD-L1 (B7-H1

UniProt

Q9EP73

途径

Cancer Immune Checkpoints