Factor VII ELISA 试剂盒
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- 抗原 See all Factor VII (F7) ELISA试剂盒
- Factor VII (F7) (Coagulation Factor VII (F7))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Mouse Coagulation Factor VII/F7 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Mouse Coagulation Factor VII.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product F7 ELISA Kit
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Coagulation Factor VII (F7) ELISA Kit
F7 适用: 小鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma
Coagulation Factor VII (F7) ELISA KitF7 适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma
Coagulation Factor VII (F7) ELISA KitF7 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Coagulation Factor VII (F7) ELISA KitF7 适用: 人 Colorimetric Sandwich ELISA 9.4 pg/mL - 600 pg/mL Plasma
Coagulation Factor VII (F7) ELISA KitF7 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Coagulation Factor VII (F7) ELISA KitF7 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Coagulation Factor VII (F7) ELISA KitF7 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Coagulation Factor VII (F7) ELISA KitF7 适用: 小鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Coagulation Factor VII (F7) ELISA KitF7 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Coagulation Factor VII (F7) ELISA KitF7 适用: 兔 Colorimetric Competition ELISA 3.9 ng/mL - 250 ng/mL Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all Factor VII (F7) ELISA试剂盒
- Factor VII (F7) (Coagulation Factor VII (F7))
- 别名
- Coagulation Factor VII (F7 (F7 产品)
- 别名
- F7 ELISA Kit, wu:fb59f04 ELISA Kit, zgc:109870 ELISA Kit, SPCA ELISA Kit, AI114908 ELISA Kit, ATIII ELISA Kit, At-3 ELISA Kit, At3 ELISA Kit, LOC100223776 ELISA Kit, AI132620 ELISA Kit, Cf7 ELISA Kit, FVII ELISA Kit, mfVII ELISA Kit, f7 ELISA Kit, coagulation factor VII ELISA Kit, serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1 ELISA Kit, coagulation factor 7 (serum prothrombin conversion accelerator) S homeolog ELISA Kit, F7 ELISA Kit, f7 ELISA Kit, Serpinc1 ELISA Kit, CpipJ_CPIJ009142 ELISA Kit, CpipJ_CPIJ010295 ELISA Kit, CpipJ_CPIJ020127 ELISA Kit, fa7 ELISA Kit, f7.S ELISA Kit
- UniProt
- P70375
- 途径
- Response to Growth Hormone Stimulus, Platelet-derived growth Factor Receptor Signaling
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