Factor VII ELISA 试剂盒
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Quick Overview for Factor VII ELISA 试剂盒 (ABIN8055799)
抗原
See all Factor VII (F7) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.78 ng/mL
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原理
- Sandwich Human F7 ELISA Kit to quantitate Human F7 in cell culture supernatants, serum and plasma (heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: A39-P444Capture antibody and Detection antibody: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 20 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Coagulation Factor VII (F7) ELISA Kit
F7 适用: 小鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma
Coagulation Factor VII (F7) ELISA KitF7 适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma
Coagulation Factor VII (F7) ELISA KitF7 适用: 人 Colorimetric Sandwich ELISA 9.4 pg/mL - 600 pg/mL Plasma
Coagulation Factor VII (F7) ELISA KitF7 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Coagulation Factor VII (F7) ELISA KitF7 适用: 兔 Colorimetric Competition ELISA 3.9 ng/mL - 250 ng/mL Plasma, Serum, Tissue Homogenate
Coagulation Factor VII (F7) ELISA KitF7 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Coagulation Factor VII (F7) ELISA KitF7 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Coagulation Factor VII (F7) ELISA KitHigh Sensitivity F7 适用: 小鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma
Coagulation Factor VII (F7) ELISA KitF7 适用: 小鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma
Coagulation Factor VII (F7) ELISA KitF7 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 5.9%, 7.1%, 6.4%
Inter-Assay Precision (CV%): 5.6%, 5.1%, 5.9% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- Factor VII (F7) (Coagulation Factor VII (F7))
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别名
- F7
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背景
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Background: Coagulation factor VII (EC 3.4.21.21, formerly known as proconvertin) is one of the proteins that causes blood to clot in the coagulation cascade, and in humans is coded for by the gene F7. This gene encodes coagulation factor VII which is a vitamin K-dependent factor essential for hemostasis. This factor circulates in the blood in a zymogen form, and is converted to an active form by either factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon activation of the factor VII, a heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated, and two chains are held together by a disulfide bond. In the presence of factor III and calcium ions, the activated factor then further activates the coagulation cascade by converting factor IX to factor IXa and/or factor X to factor Xa. Defects in this gene can cause coagulopathy. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides.
Gene Full Name: coagulation factor VII
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基因ID
- 2155
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UniProt
- P08709
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途径
- Response to Growth Hormone Stimulus, Platelet-derived growth Factor Receptor Signaling
抗原 See all Factor VII (F7) ELISA试剂盒
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