2B4 ELISA 试剂盒
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- 抗原 See all 2B4 (CD244) ELISA试剂盒
- 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))
- 适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Mouse 2B4/CD244/SLAMF4 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Mouse 2B4.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Natural Killer Cell Receptor 2B4 (CD244) ELISA Kit
CD244 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Natural Killer Cell Receptor 2B4 (CD244) ELISA KitCD244 适用: 人 AA 22-221 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Natural Killer Cell Receptor 2B4 (CD244) ELISA KitCD244 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Natural Killer Cell Receptor 2B4 (CD244) ELISA KitCD244 适用: 人 Colorimetric Sandwich ELISA 0.625-40 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all 2B4 (CD244) ELISA试剂盒
- 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))
- 别名
- 2B4 (CD244/SLAMF4) (CD244 产品)
- 别名
- CD244 ELISA Kit, 2B4 ELISA Kit, NAIL ELISA Kit, NKR2B4 ELISA Kit, Nmrk ELISA Kit, SLAMF4 ELISA Kit, C9.1 ELISA Kit, F730046O15Rik ELISA Kit, Ly90 ELISA Kit, CD244 molecule ELISA Kit, CD244 natural killer cell receptor 2B4 ELISA Kit, CD244 ELISA Kit, Cd244 ELISA Kit
- UniProt
- Q07763
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