2B4 ELISA 试剂盒
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Quick Overview for 2B4 ELISA 试剂盒 (ABIN1672841)
抗原
See all 2B4 (CD244) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 15.6 pg/mL
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原理
- Sandwich Human CD244/2B4 ELISA Kit to quantitate Human CD244 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: C22-R221Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Natural Killer Cell Receptor 2B4 (CD244) ELISA Kit
CD244 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Natural Killer Cell Receptor 2B4 (CD244) ELISA KitCD244 适用: 人 Colorimetric Sandwich ELISA 0.625-40 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Natural Killer Cell Receptor 2B4 (CD244) ELISA KitCD244 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 4.8%, 5.7%, 4.6%
Inter-Assay Precision (CV%): 5.1%, 5.9%, 6.4% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))
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别名
- CD244
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背景
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Background: CD244 (Cluster of Differentiation 244) is a human protein encoded by the CD244 gene. It is also known as Natural Killer Cell Receptor 2B4. Members of the CD2 family adhere to each other instead,the cell surface glycoprotein 2B4 is related to CD2 and is implicated in the regulation of NK- and T-cell function. The 2B4 gene was mapped to 1q22 on gene map, where SLAM, CD48, CD84 and LY9 are also located. Using recombinant human NK cell-activating ligand 2B4 fused to domains 3 and 4 of rodent Cd4 and flow cytometric analysis, It was found that CD48 binds to 2B4. BIAcore surface plasmon resonance analysis showed that the affinity of CD48 for 2B4 is approximately 10-fold higher than that shown for CD48 and CD2.
Gene Full Name: CD244 Molecule
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基因ID
- 51744
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UniProt
- Q9BZW8
抗原 See all 2B4 (CD244) ELISA试剂盒
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