S100A9 ELISA 试剂盒
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Quick Overview for S100A9 ELISA 试剂盒 (ABIN6730139)
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See all S100A9 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 13 pg/mL
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原理
- Mouse S100A9 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
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Analytical Method
- Quantitative
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特异性
- This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse aFGF, ANG-2, APCS, beta-NGF, BMP-5, BMPR-II, CD164, CHST4, CHST7, CRELD1, FCRN, FGF R3, GAPDH, GDF11, IFNAR1, IFN-beta, IL-2 Rg, LAG-3, Mer, MMP-8, MOG, Neuroplastin, PAI-1, RBP4, Ryk, S100A1, S100A6, SEMA6A, Serpin F1, Siglec-1, TCAM-1, Thrombomodulin, Tie-2, TIM-4, TIMP-4, TLR1, TSP-4, VLDL R, VSIG4.
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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S100 Calcium Binding Protein A9 (S100A9) ELISA Kit
S100A9 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A9 (S100A9) ELISA KitS100A9 适用: 人 Colorimetric Sandwich ELISA 4.69 ng/mL - 300 ng/mL Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A9 (S100A9) ELISA KitS100A9 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A9 (S100A9) ELISA KitS100A9 适用: 小鼠 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A9 (S100A9) ELISA KitS100A9 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A9 (S100A9) ELISA KitS100A9 适用: Cow Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
S100 Calcium Binding Protein A9 (S100A9) ELISA KitS100A9 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A9 (S100A9) ELISA KitS100A9 适用: Cow Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A9 (S100A9) ELISA KitS100A9 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A9 (S100A9) ELISA KitS100A9 适用: 人 Colorimetric Sandwich ELISA 4.69-300 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Suggested Plasma Dilution: 2 fold
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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有效期
- 6 months
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- S100A9 (S100 Calcium Binding Protein A9 (S100A9))
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别名
- S100A9
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背景
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Gene Symbols: S100A9, Cagb, Mrp14
Protein Name (Synonyms): Protein S100-A9 (Calgranulin-B) (Leukocyte L1 complex heavy chain) (Migration inhibitory factor-related protein 14) (MRP-14) (p14) (S100 calcium-binding protein A9)
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基因ID
- 20202
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UniProt
- P31725
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途径
- Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins
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