S100A9 ELISA 试剂盒
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Quick Overview for S100A9 ELISA 试剂盒 (ABIN2859311)
抗原
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适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.2 pg/mL
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原理
- Sandwich Mouse S100A9 ELISA Kit to quantitate Mouse S100a9 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: A2-K113Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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S100 Calcium Binding Protein A9 (S100A9) ELISA Kit
S100A9 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A9 (S100A9) ELISA KitS100A9 适用: 人 Colorimetric Sandwich ELISA 4.69 ng/mL - 300 ng/mL Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A9 (S100A9) ELISA KitS100A9 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A9 (S100A9) ELISA KitS100A9 适用: 小鼠 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A9 (S100A9) ELISA KitS100A9 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A9 (S100A9) ELISA KitS100A9 适用: Cow Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
S100 Calcium Binding Protein A9 (S100A9) ELISA KitS100A9 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A9 (S100A9) ELISA KitS100A9 适用: 人 Colorimetric Sandwich ELISA 4.69-300 ng/mL Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A9 (S100A9) ELISA KitS100A9 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A9 (S100A9) ELISA KitS100A9 适用: Cow Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 4.3%, 5.5%, 4.9%
Inter-Assay Precision (CV%): 5.6%, 7%, 5.8% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- S100A9 (S100 Calcium Binding Protein A9 (S100A9))
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别名
- S100A9
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背景
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Background: Protein S100A9 also known as migration inhibitory factor-related protein 14 (MRP-14) or calgranulin-B is a protein that is encoded by the S100A9 gene. S100A9 is a member of the S100 family of proteins containing 2 EF hand calcium-binding motifs. It is mapped to Chromosome 3 in mouse. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. This protein may function in the inhibition of casein kinase. S100A9 complexes with S100A8, another member of the S100 family of calcium-modulated proteins regulates myeloid cell function by binding to Toll-like receptor-4 (TLR-4) and the receptor for advanced glycation end products.
Gene Full Name: S100 calcium binding protein A9 (calgranulin B)
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基因ID
- 20202
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UniProt
- P31725
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途径
- Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins
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