APOC1 ELISA 试剂盒
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- 抗原 See all APOC1 ELISA试剂盒
- APOC1 (Apolipoprotein C-I (APOC1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human ApoC1 ELISA Kit. The SpeedELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of target protein in biological sample such as serum, plasma, and cell culture supernatants.
- 品牌
- SpeedELISA
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human ApoC1. Other species not determined yet.
- 产品特性
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- Compressed workflow with only one wash step and three incubation steps
- 3 hours total processing time
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Streptavidin-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Capture Antibody
- HRP Conjugated Detection Antibody
- TMB One-Step Substrate
- 试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm.
- Precision pipettes to deliver 2 µl to 1 ml volumes.
- Adjustable 1-25 ml pipettes for reagent preparation.
- 100 ml and 1 liter graduated cylinders.
- Absorbent paper.
- Distilled or deionized water.
- Log-log graph paper or computer and software for ELISA data analysis.
- Tubes to prepare standard or sample dilutions.
- Top Product
- Discover our top product APOC1 ELISA Kit
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- 实验时间
- 3 h
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 70 µl of the Antibody Cocktail to each well.
- Incubate 10 minutes at RT.
- Add 50 µl of sample or standard to each well.
- Incubate 2 h at RT.
- Wash Wells
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment.Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C. For prepared reagent storage, see table below.
- 有效期
- 6 months
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- 抗原 See all APOC1 ELISA试剂盒
- APOC1 (Apolipoprotein C-I (APOC1))
- 别名
- ApoC1 (APOC1 产品)
- 别名
- Apo-CIB ELISA Kit, ApoC-IB ELISA Kit, ALPCI ELISA Kit, LRRG04 ELISA Kit, APOC1 ELISA Kit, apoc1 ELISA Kit, apolipoprotein C1 ELISA Kit, apolipoprotein C-I ELISA Kit, Apolipoprotein C-I ELISA Kit, APOC1 ELISA Kit, Apoc1 ELISA Kit, apoc1 ELISA Kit
- 途径
- Apoptosis
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