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APOC1 ELISA 试剂盒

APOC1 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN6730064
发货至: 中国
  • 抗原 See all APOC1 ELISA试剂盒
    APOC1 (Apolipoprotein C-I (APOC1))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human ApoC1 ELISA Kit. The SpeedELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of target protein in biological sample such as serum, plasma, and cell culture supernatants.
    品牌
    SpeedELISA
    样品类型
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes Human ApoC1. Other species not determined yet.
    产品特性
    • Compressed workflow with only one wash step and three incubation steps
    • 3 hours total processing time
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Streptavidin-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Capture Antibody
    • HRP Conjugated Detection Antibody
    • TMB One-Step Substrate
    试剂未包括
    1. Microplate reader capable of measuring absorbance at 450 nm.
    2. Precision pipettes to deliver 2 µl to 1 ml volumes.
    3. Adjustable 1-25 ml pipettes for reagent preparation.
    4. 100 ml and 1 liter graduated cylinders.
    5. Absorbent paper.
    6. Distilled or deionized water.
    7. Log-log graph paper or computer and software for ELISA data analysis.
    8. Tubes to prepare standard or sample dilutions.
    Top Product
    Discover our top product APOC1 ELISA Kit
  • 实验时间
    3 h
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 70 µl of the Antibody Cocktail to each well.
    3. Incubate 10 minutes at RT.
    4. Add 50 µl of sample or standard to each well.
    5. Incubate 2 h at RT.
    6. Wash Wells
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment.Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C. For prepared reagent storage, see table below.
    有效期
    6 months
  • 抗原 See all APOC1 ELISA试剂盒
    APOC1 (Apolipoprotein C-I (APOC1))
    别名
    ApoC1 (APOC1 产品)
    别名
    Apo-CIB ELISA Kit, ApoC-IB ELISA Kit, ALPCI ELISA Kit, LRRG04 ELISA Kit, APOC1 ELISA Kit, apoc1 ELISA Kit, apolipoprotein C1 ELISA Kit, apolipoprotein C-I ELISA Kit, Apolipoprotein C-I ELISA Kit, APOC1 ELISA Kit, Apoc1 ELISA Kit, apoc1 ELISA Kit
    途径
    Apoptosis
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