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APOC1 ELISA 试剂盒

This 人 APOC1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 APOC1.

产品编号 ABIN6730064

发货至: 中国

Quick Overview for APOC1 ELISA 试剂盒 (ABIN6730064)

抗原

See all APOC1 ELISA试剂盒

APOC1 (Apolipoprotein C-I (APOC1))

适用

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人

检测方法

Colorimetric

实验类型

Sandwich ELISA

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma, Serum

Discover our top product APOC1 ELISA Kit

原理

Human ApoC1 ELISA Kit. The SpeedELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of target protein in biological sample such as serum, plasma, and cell culture supernatants.

品牌

SpeedELISA

Analytical Method

Quantitative

特异性

This ELISA antibody pair recognizes Human ApoC1. Other species not determined yet.

产品特性
- Compressed workflow with only one wash step and three incubation steps
- 3 hours total processing time
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
组件
- Streptavidin-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Capture Antibody
- HRP Conjugated Detection Antibody
- TMB One-Step Substrate
试剂未包括
1. Microplate reader capable of measuring absorbance at 450 nm.
2. Precision pipettes to deliver 2 µl to 1 ml volumes.
3. Adjustable 1-25 ml pipettes for reagent preparation.
4. 100 ml and 1 liter graduated cylinders.
5. Absorbent paper.
6. Distilled or deionized water.
7. Log-log graph paper or computer and software for ELISA data analysis.
8. Tubes to prepare standard or sample dilutions.
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APOC1 适用: Cow Colorimetric Competition ELISA Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

实验时间

3 h

实验流程
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 70 µl of the Antibody Cocktail to each well.
3. Incubate 10 minutes at RT.
4. Add 50 µl of sample or standard to each well.
5. Incubate 2 h at RT.
6. Wash Wells
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.
限制

仅限研究用
储存方法

The entire kit may be stored at -20°C for up to 1 year from the date of shipment.Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C. For prepared reagent storage, see table below.

有效期

6 months
抗原 See all APOC1 ELISA试剂盒

APOC1 (Apolipoprotein C-I (APOC1))

别名

ApoC1

途径

Apoptosis

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