M-CSF/CSF1 ELISA 试剂盒
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Quick Overview for M-CSF/CSF1 ELISA 试剂盒 (ABIN6720397)
抗原
See all M-CSF/CSF1 (CSF1) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 62.5 pg/mL
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Supplier Product No.
- koa0253
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Supplier
- Rockland
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原理
- Human M-CSF Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: E33-S190 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Colony Stimulating Factor 1 (Macrophage) (CSF1) ELISA Kit
CSF1 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate, Urine
Colony Stimulating Factor 1 (Macrophage) (CSF1) ELISA KitCSF1 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 1 (Macrophage) (CSF1) ELISA KitCSF1 适用: 猴 Colorimetric Sandwich ELISA 39.07 pg/mL - 2500 pg/mL Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 1 (Macrophage) (CSF1) ELISA KitCSF1 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 1 (Macrophage) (CSF1) ELISA KitCSF1 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 1 (Macrophage) (CSF1) ELISA KitCSF1 适用: 小鼠 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 1 (Macrophage) (CSF1) ELISA KitCSF1 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 1 (Macrophage) (CSF1) ELISA KitSmall Sample CSF1 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate, Urine
Colony Stimulating Factor 1 (Macrophage) (CSF1) ELISA KitCSF1 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 1 (Macrophage) (CSF1) ELISA KitCSF1 适用: 人 Colorimetric Sandwich ELISA 5 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
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说明
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Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human M-CSF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, tissue lysates, serum, plasma (heparin or EDTA), saliva or urine to each empty well. It is recommended that each human M-CSF standard solution and each sample be measured in duplicate.
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实验精密度
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Intra-Assay Precision (CV%): 5.3%, 4.8%, 4.6%
Inter-Assay Precision (CV%): 6.5%, 5.6%, 4.6% -
限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))
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别名
- M-CSF
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背景
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Background: M-CSF, also called CSF1, has a role in development of the placenta. Uterine CSF1 concentration is regulated by a synergistic action of estradiol and progesterone. CSF1 is produced by uterine glandular epithelial cells. It had been found that FMS, the CSF1 receptor, is expressed in placenta and choriocarcinoma cell lines1. The CSF1 gene is mapped to 1p21-p13 and contains 10 exons and 9 introns spanning 20 kb2. And there are 2 forms of CSF1, with 224 and 522 amino acids, resulting from alternative splicing3.
Gene Full Name: colony stimulating factor 1
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基因ID
- 1435
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NCBI登录号
- NP_000748
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UniProt
- P09603
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途径
- RTK signaling
抗原 See all M-CSF/CSF1 (CSF1) ELISA试剂盒
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