FURIN ELISA 试剂盒
Quick Overview for FURIN ELISA 试剂盒 (ABIN6720393)
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检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 312 pg/mL
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Supplier Product No.
- koa0553
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Supplier
- Rockland
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原理
- Human Furin Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant human Furin. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN) ELISA Kit
FURIN 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN) ELISA KitFURIN 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN) ELISA KitFURIN 适用: 小鼠 Colorimetric Sandwich ELISA 46.875 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN) ELISA KitFURIN 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN) ELISA KitFURIN 适用: 人 AA 108-715 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human Furin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, tissue homogenates or cell lysates to each empty well. It is recommended that each human Furin standard solution and each sample be measured in duplicate.
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说明
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Standard: Expression system for standard: NSO, Immunogen sequence: D108-E715
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- FURIN (Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN))
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别名
- Furin
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背景
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Synonyms: Dibasic processing enzyme, Dibasic-processing enzyme, FES upstream region, FUR, FURIN, Furin membrane associated receptor protein, FURIN_HUMAN, PACE, Paired basic amino acid residue cleaving enzyme, Paired basic amino acid residue-cleaving enzyme, PCSK3, Proprotein convertase subtilisin/kexin type 3, SPC1
Background: Furin (Fur), also known as PACE, is a protein that in humans is encoded by the FURIN gene. The protein encoded by this gene is an enzyme which belongs to the subtilisin-like proprotein convertase family. The FUR gene is located approximately 1 kb upstream of the FES gene on chromosome 15q25-q26. This gene is thought to play a role in tumor progression. Furin is one of the proteases responsible for the proteolytic cleavage of HIV envelope polyprotein precursor gp160 to gp120 and gp41 prior to viral assembly. Furin is enriched in the Golgi apparatus, where it functions to cleave other proteins into their mature/active forms. Expression of furin in T-cells is required for maintenance of peripheral immune tolerance.
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基因ID
- 5045
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NCBI登录号
- NP_001276752
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UniProt
- P09958
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途径
- Notch Signaling, Neurotrophin Signaling Pathway
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