DLL1 ELISA 试剂盒
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北京 101111
Quick Overview for DLL1 ELISA 试剂盒 (ABIN6720373)
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See all DLL1 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 78 pg/mL
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Supplier Product No.
- koa0752
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Supplier
- Rockland
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原理
- Human DLL1 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: S22-G540 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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delta-Like 1 (DLL1) ELISA Kit
DLL1 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
delta-Like 1 (DLL1) ELISA KitDLL1 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
delta-Like 1 (DLL1) ELISA KitDLL1 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
delta-Like 1 (DLL1) ELISA KitDLL1 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
delta-Like 1 (DLL1) ELISA KitDLL1 适用: 大鼠 AA 18-531 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
delta-Like 1 (DLL1) ELISA KitDLL1 适用: 小鼠 AA 18-535 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
delta-Like 1 (DLL1) ELISA KitDLL1 适用: 人 AA 22-540 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Serum, Urine
delta-Like 1 (DLL1) ELISA KitDLL1 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Plasma, Serum, Tissue Homogenate
delta-Like 1 (DLL1) ELISA KitDLL1 适用: 人 Colorimetric Sandwich ELISA 18.75-1200 pg/mL Plasma, Serum, Tissue Homogenate
delta-Like 1 (DLL1) ELISA KitDLL1 适用: 人 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma, Serum, Urine
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说明
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Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL human DLL1 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), urine or human milk to each empty well. We recommend that each human DLL1 standard solution and each sample is measured in duplicate.
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实验精密度
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Intra-Assay Precision (CV%): 4.4%, 4.3%, 4%
Inter-Assay Precision (CV%): 4.6%, 4.7%, 5.5% -
限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- DLL1 (delta-Like 1 (DLL1))
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别名
- DLL1
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背景
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Background: DLL1 (DELTA-LIKE 1), also known as DL1 or DELTA1, is a protein that in humans is encoded by the DLL1 gene. DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis, and it is also important in cell-to-cell communication. This gene is mapped to chromosome 6q27, near a locus for type I diabetes. Functional analysis suggested that a soluble fusion protein containing the DSL domain of DLL1 and its adjacent 50 N-terminal amino acids increased the viability of hemopoietic cells but inhibited cell death. It has been found that DLL1 blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.
Gene Full Name: delta like canonical Notch ligand 1
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基因ID
- 28514
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NCBI登录号
- NP_005609
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UniProt
- O00548
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途径
- Notch Signaling, Stem Cell Maintenance
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