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Amphiregulin ELISA 试剂盒

AREG 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Breast Milk, Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum
产品编号 ABIN6720359
发货至: 中国
  • 抗原 See all Amphiregulin (AREG) ELISA试剂盒
    Amphiregulin (AREG)
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    15.6 pg/mL - 1000 pg/mL
    最低检测浓度
    15.6 pg/mL
    应用范围
    ELISA
    原理
    Sandwich ELISA for Quantitative Detection of Antigen
    样品类型
    Breast Milk, Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum
    Analytical Method
    Quantitative
    产品特性

    Synonyms: A REG, AR, AREG, Colorectum cell derived growth

    Background: Amphiregulin, also known as AREG, is a protein that in humans is encoded by the AREG gene. The protein encoded by this gene is a member of the epidermal growth factor family. This gene is mapped to 4q13.1. It is an autocrine growth factor as well as a mitogen forastrocytes, Schwann cells, fibroblasts. Amphiregulin is related to toepidermal growth factor(EGF) and transforming growth factor alpha(TGF-alpha). This protein interacts with the Epidermal growth factor receptor to promote the growth of normal epithelial cells. It has been found that transgene integration and subsequent expression of Amphiregulin in basal keratinocytes correlated with a psoriasis-like skin phenotype, and its expression was increased in parallel with typical Th2 cytokines. Amphiregulin is also an important paracrine mediator of estrogen function specifically required for puberty-induced ductal elongation but not for any earlier or later developmental stages, and it can enhance resistance to nematodes.

    Gene Name: AREG

    Production: Natural and recombinant human Amphiregulin. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: E.coli, Immunogen sequence: S101-K198

    Top Product
    Discover our top product AREG ELISA Kit
  • 应用备注

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human Amphiregulin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), saliva or human milk to each empty well. It is recommended that each human Amphiregulin standard solution and each sample be measured in duplicate.

    Blood Product Anticoagulant: Heparin Sodium

    ELISA Dilution: 15.6pg/mL-1000pg/mL

    样本量
    100 μL
    板类型
    Pre-coated
    限制
    仅限研究用
  • 储存条件
    RT,4 °C,-20 °C
    储存方法
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • 抗原 See all Amphiregulin (AREG) ELISA试剂盒
    Amphiregulin (AREG)
    别名
    Amphiregulin(AR) (AREG 产品)
    别名
    AR ELISA Kit, CRDGF ELISA Kit, SDGF ELISA Kit, Sdgf ELISA Kit, AREG ELISA Kit, AREGB ELISA Kit, AREG/SDGF ELISA Kit, amphiregulin ELISA Kit, AREG ELISA Kit, Areg ELISA Kit
    基因ID
    374
    NCBI登录号
    NP_001648
    UniProt
    P15514
    途径
    RTK signaling, EGFR Signaling Pathway
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