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Complement Factor H ELISA 试剂盒

Name: Complement Factor H ELISA 试剂盒
SKU: ABIN6720349
Availability: OutOfStock

This 人 Complement Factor H ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 Complement Factor H.

产品编号 ABIN6720349

Supplier Product No.: koa0843

发货至: 中国

Datasheet as PDF

Quick Overview for Complement Factor H ELISA 试剂盒 (ABIN6720349)

抗原

See all Complement Factor H (CFH) ELISA试剂盒

Complement Factor H (CFH)

适用

人

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

1.56 ng/mL - 100 ng/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Urine

Discover our top product CFH ELISA Kit

最低检测浓度

1.56 ng/mL

Supplier Product No.

koa0843

Supplier

Rockland

原理

Human Complement H - CFH Sandwich ELISA Kit for Quantitative Detection

品牌

AccuSignal™

Analytical Method

Quantitative

特异性

Production: Natural and recombinant human CFH. There is no detectable cross-reactivity with other relevant proteins.

灵敏度

< 50 pg/mL

组件
- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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CFH 适用: 人 AA 860-1231 Colorimetric Sandwich ELISA 1.56-100 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine

应用备注

Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL human CFH standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. It is recommended that each human CFH standard solution and each sample be measured in duplicate.

说明

Standard: Expression system for standard: NSO, Immunogen sequence: S860-R1231

样本量

100 μL

板类型

Pre-coated

限制

仅限研究用
储存条件

4 °C,-20 °C

储存方法

Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

有效期

12 months
抗原 See all Complement Factor H (CFH) ELISA试剂盒

Complement Factor H (CFH)

别名

Complement H/CFH

背景

Synonyms: adrenomedullin binding protein, age related maculopathy susceptibility 1, AHUS 1, AHUS1, AMBP 1, AMBP1, ARMD 4, ARMD4, ARMS 1, ARMS1, beta 1 H globulin, beta 1H, beta1H, CFAH_HUMAN, CFH, CFHL 3, CFHL3, Complement factor H, complement factor H isoform b, Factor H, factor H like 1, FH, FHL 1, FHL1, H factor 1 (complement)|H factor 1, H factor 2 (complement), HF 1, HF 2, HF, HF1, HF2, HUS, MGC88246
Background: Complement factor H (CFH), originally known as beta-1H globulin, is a serum glycoprotein that regulates the function of the alternative complement pathway in fluid phase and on cellular surfaces. It binds to C3b, accelerates the decay of the alternative pathway convertase C3bBb, and also acts as a cofactor for complement factor I, another C3b inhibitor. The CFH gene is located on chromosome 1q32-q32.1 within a cluster of genes encoding the regulatory complement components of the activation of C3 (RCA for 'regulators of complement activation'). This gene cluster includes decay-accelerating factor (DAF), C4-binding protein (C4BPA and C4BPB), and the factor H-related genes CFHR1, CFHR2, CFHR3, CFHR4, and CFHR5, among others. The gene family has arisen by multiple duplication events.

基因ID

3075

NCBI登录号

NP_000177

UniProt

P08603

途径

Complement System, Cellular Response to Molecule of Bacterial Origin