RBP4 ELISA 试剂盒
Quick Overview for RBP4 ELISA 试剂盒 (ABIN6720318)
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检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 312 pg/mL
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Supplier Product No.
- koa0498
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Supplier
- Rockland
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原理
- Human RBP4 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant human RBP4. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Retinol Binding Protein 4, Plasma (RBP4) ELISA Kit
RBP4 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Retinol Binding Protein 4, Plasma (RBP4) ELISA KitRBP4 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Retinol Binding Protein 4, Plasma (RBP4) ELISA KitRBP4 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Retinol Binding Protein 4, Plasma (RBP4) ELISA KitRBP4 适用: 大鼠 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Retinol Binding Protein 4, Plasma (RBP4) ELISA KitRBP4 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Serum
Retinol Binding Protein 4, Plasma (RBP4) ELISA KitRBP4 适用: 小鸡 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Retinol Binding Protein 4, Plasma (RBP4) ELISA KitRBP4 适用: Cow Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Retinol Binding Protein 4, Plasma (RBP4) ELISA KitRBP4 适用: Pig Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Retinol Binding Protein 4, Plasma (RBP4) ELISA KitRBP4 适用: 人 AA 19-201 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine
Retinol Binding Protein 4, Plasma (RBP4) ELISA KitRBP4 适用: 人 Colorimetric Competition ELISA 15.6-1000 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human RBP4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin, EDTA) or urine to each empty well. We recommend that each human RBP4 standard solution and each sample is measured in duplicate.
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说明
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Standard: Expression system for standard: NSO, Immunogen sequence: E19-L201
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- RBP4 (Retinol Binding Protein 4, Plasma (RBP4))
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别名
- RBP4
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背景
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Synonyms: Plasma retinol binding protein, Plasma retinol-binding protein, Plasma retinol-binding protein(1-176), PRBP, PRO2222, RBP, RBP4, RDCCAS, RET4_HUMAN, Retinol binding protein 4, Retinol binding protein 4 interstitial, Retinol binding protein 4 plasma
Background: Retinol binding protein 4, plasma, also known as RBP4, belongs to the lipocalin family and is the specific carrier for retinol(vitamin A alcohol) in the blood. It is protein that in humans is encoded by the RBP4 gene. RBP4 gene resides just centromeric of the cluster of CYP2C genes on 10q24. The mouse Rbp4 locus is closely linked and just proximal to the locus for phenobarbital-inducible cytochrome P450-2c(Cyp-2c) at the distal end of chromosome 19. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein post translationally and results in defective delivery and supply to the epidermal cells. The standard used in this kit is recombinant protein, with E19-L201 aa sequence, the molecular weight is 22kda.
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基因ID
- 5950
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NCBI登录号
- NP_006735
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UniProt
- P02753
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途径
- Regulatory RNA Pathways, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Production of Molecular Mediator of Immune Response
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