RBP4 ELISA 试剂盒
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- 抗原 See all RBP4 ELISA试剂盒
- RBP4 (Retinol Binding Protein 4, Plasma (RBP4))
- 抗原表位
- AA 19-201
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 312-20000 pg/mL
- 最低检测浓度
- 312 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human RBP4
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: E19-L201 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: NSO
Immunogen sequence: E19-L201 - Featured
- Discover our best selling RBP4 ELISA Kit
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- Discover our top product RBP4 ELISA Kit
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Retinol Binding Protein 4, Plasma (RBP4) ELISA Kit
RBP4 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Retinol Binding Protein 4, Plasma (RBP4) ELISA KitRBP4 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Retinol Binding Protein 4, Plasma (RBP4) ELISA KitRBP4 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Retinol Binding Protein 4, Plasma (RBP4) ELISA KitRBP4 适用: 大鼠 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Retinol Binding Protein 4, Plasma (RBP4) ELISA KitRBP4 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Serum
Retinol Binding Protein 4, Plasma (RBP4) ELISA KitRBP4 适用: 小鸡 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Retinol Binding Protein 4, Plasma (RBP4) ELISA KitRBP4 适用: Pig Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Retinol Binding Protein 4, Plasma (RBP4) ELISA KitRBP4 适用: Cow Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Retinol Binding Protein 4, Plasma (RBP4) ELISA KitRBP4 适用: 人 Colorimetric Competition ELISA 15.6-1000 ng/mL Plasma, Serum, Tissue Homogenate
Retinol Binding Protein 4, Plasma (RBP4) ELISA KitRBP4 适用: 小鼠 AA 19-201 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the calycin superfamily. Lipocalin family.
- 板类型
- Pre-coated
- 实验流程
- human RBP4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for RBP4 has been precoated onto 96-well plates. Standards(NSO, E19-L201) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for RBP4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human RBP4 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human RBP4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human RBP4 standard solution and each sample is measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 2.8, Standard deviation: 0.174, CV(%): 6.2
- Sample 2: n=16, Mean(ng/ml): 5, Standard deviation: 0.295, CV(%): 5.9
- Sample 3: n=16, Mean(ng/ml): 9.43, Standard deviation: 0.434, CV(%): 4.6,
- Sample 1: n=24, Mean(ng/ml): 3.4, Standard deviation: 0.255, CV(%): 7.5
- Sample 2: n=24, Mean(ng/ml): 6.2, Standard deviation: 0.422, CV(%): 6.8
- Sample 3: n=24, Mean(ng/ml): 11.5, Standard deviation: 0.874, CV(%): 7.6
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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: "Vitreous levels of apolipoprotein A1 and retinol binding protein 4 in human rhegmatogenous retinal detachment associated with choroidal detachment." in: Molecular vision, Vol. 24, pp. 252-260, (2018) (PubMed).
: "Effect of Omega-3 Supplementation on Lipocalin 2 and Retinol-Binding Protein 4 in Type 2 Diabetic Patients." in: Iranian journal of public health, Vol. 45, Issue 2, pp. 179-85, (2016) (PubMed).
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: "Vitreous levels of apolipoprotein A1 and retinol binding protein 4 in human rhegmatogenous retinal detachment associated with choroidal detachment." in: Molecular vision, Vol. 24, pp. 252-260, (2018) (PubMed).
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- 抗原 See all RBP4 ELISA试剂盒
- RBP4 (Retinol Binding Protein 4, Plasma (RBP4))
- 别名
- RBP4 (RBP4 产品)
- 别名
- RDCCAS ELISA Kit, RBP ELISA Kit, RNBP ELISA Kit, rbp4 ELISA Kit, MGC54038 ELISA Kit, srbp ELISA Kit, PRBP ELISA Kit, RBPA ELISA Kit, Rbp-4 ELISA Kit, fb23c12 ELISA Kit, rbp ELISA Kit, wu:fb23c12 ELISA Kit, wu:fb58d04 ELISA Kit, wu:fb72b04 ELISA Kit, zgc:86911 ELISA Kit, retinol binding protein 4 ELISA Kit, renin binding protein ELISA Kit, retinol binding protein 4 S homeolog ELISA Kit, retinol binding protein 4 A, plasma ELISA Kit, retinol binding protein 4, plasma ELISA Kit, retinol binding protein 4 L homeolog ELISA Kit, RBP4 ELISA Kit, RENBP ELISA Kit, rbp4.S ELISA Kit, rbp4 ELISA Kit, Rbp4 ELISA Kit, RBP4A ELISA Kit, rbp4.L ELISA Kit
- 背景
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Protein Function: Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli.
Background: Retinol binding protein 4, plasma, also known as RBP4, belongs to the lipocalin family and is the specific carrier for retinol(vitamin A alcohol) in the blood. It is protein that in humans is encoded by the RBP4 gene. RBP4 gene resides just centromeric of the cluster of CYP2C genes on 10q24. The mouse Rbp4 locus is closely linked and just proximal to the locus for phenobarbital-inducible cytochrome P450-2c(Cyp-2c) at the distal end of chromosome 19. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. The standard used in this kit is recombinant protein, with E19-L201 aa sequence, the molecular weight is 22kda.
Synonyms: Retinol-binding protein 4,Plasma retinol-binding protein,PRBP,RBP,Plasma retinol-binding protein(1-182),Plasma retinol-binding protein(1-181),Plasma retinol-binding protein(1-179),Plasma retinol-binding protein(1-176),RBP4,PRO2222,
Full Gene Name: Retinol-binding protein 4
Cellular Localisation: Secreted. - 基因ID
- 5950
- UniProt
- P02753
- 途径
- Regulatory RNA Pathways, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Production of Molecular Mediator of Immune Response
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