SERPINC1 ELISA 试剂盒
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Quick Overview for SERPINC1 ELISA 试剂盒 (ABIN6720315)
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See all SERPINC1 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 312 pg/mL
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Supplier Product No.
- koa0839
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Supplier
- Rockland
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原理
- Human Serpin C1 - Antithrombin-III Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: H33-K464 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Serpin Family C Member 1 (SERPINC1) ELISA Kit
SERPINC1 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 ng/mL - 1000 ng/mL Plasma
Serpin Family C Member 1 (SERPINC1) ELISA KitSERPINC1 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma
Serpin Family C Member 1 (SERPINC1) ELISA KitSERPINC1 适用: 大鼠 Colorimetric Sandwich ELISA 39 ng/mL - 2500 ng/mL Plasma, Serum, Tissue Homogenate
Serpin Family C Member 1 (SERPINC1) ELISA KitSERPINC1 适用: 人 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Tissue Homogenate
Serpin Family C Member 1 (SERPINC1) ELISA KitSERPINC1 适用: 大鼠 Colorimetric Sandwich ELISA 12.5 ng/mL - 400 ng/mL Plasma, Serum, Urine
Serpin Family C Member 1 (SERPINC1) ELISA KitSERPINC1 适用: 小鼠 Colorimetric Sandwich ELISA 0.94 ng/mL - 60 ng/mL Plasma, Serum
Serpin Family C Member 1 (SERPINC1) ELISA KitSERPINC1 适用: 人 AA 33-464 Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum, Urine
Serpin Family C Member 1 (SERPINC1) ELISA KitSERPINC1 适用: 小鼠 Colorimetric Sandwich ELISA 0.937 ng/mL - 60 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Serpin Family C Member 1 (SERPINC1) ELISA KitSERPINC1 适用: 小鼠 Colorimetric Sandwich ELISA 3.125-100 ng/mL Cell Culture Cells, Plasma, Serum, Urine
Serpin Family C Member 1 (SERPINC1) ELISA KitSERPINC1 适用: 人 Colorimetric Competition ELISA 0.063-4 μg/mL Plasma, Serum
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说明
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Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human Serpin C1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin, EDTA, citrate) or urine to each empty well. We recommend that each human Serpin C1 standard solution and each sample is measured in duplicate.
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实验精密度
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Intra-Assay Precision (CV%): 4.4%, 7.8%, 4.2%
Inter-Assay Precision (CV%): 5.3%, 8.5%, 5.9% -
限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- SERPINC1 (Serpin Family C Member 1 (SERPINC1))
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别名
- Serpin C1/Antithrombin-III
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背景
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Background: Antithrombin III is the most important inhibitor of thrombin and other coagulation proteinases. It belongs to the serine proteinase inhibitor (serpin) superfamily of inhibitors and structurally related proteins, which contain reactive centers that have evolved to attract and entrap certain proteinases. Inherited antithrombin III deficiency (AT3D) is a risk factor for the early development of venous thromboembolism (THPH7). Antithrombin III regulates clot formation both by inhibiting thrombin activity ly and by interfering with earlier stages of the clotting cascade. By in situ hybridization and quantitative analysis of DNA dosage in carriers of chromosome 1 deletions, AT3 is mapped to 1q23-q25. The AT3 gene has 7 exons. It contains 9 complete and 1 partial repetitive ALU sequence elements, which occur in the introns of the gene at a higher frequency (about 22 % of the intron sequence) than in the genome as a whole (about 5 %).
Gene Full Name: serpin family C member 1
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基因ID
- 462
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NCBI登录号
- NP_000479
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UniProt
- P01008
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