SERPINC1 ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for SERPINC1 ELISA 试剂盒 (ABIN2859247)
抗原
See all SERPINC1 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 312 pg/mL
-
原理
- Sandwich Human Serpin C1/Antithrombin-III ELISA Kit to quantitate Human SERPINC1 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.
-
Analytical Method
- Quantitative
-
特异性
-
Expression system for standard: NS0
Immunogen sequence: H33-K464Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat
-
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
-
灵敏度
- < 10 pg/mL
-
组件
-
- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
-
试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
-
-
Serpin Family C Member 1 (SERPINC1) ELISA Kit
SERPINC1 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 ng/mL - 1000 ng/mL Plasma
Serpin Family C Member 1 (SERPINC1) ELISA KitSERPINC1 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma
Serpin Family C Member 1 (SERPINC1) ELISA KitSERPINC1 适用: 大鼠 Colorimetric Sandwich ELISA 39 ng/mL - 2500 ng/mL Plasma, Serum, Tissue Homogenate
Serpin Family C Member 1 (SERPINC1) ELISA KitSERPINC1 适用: 人 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum, Tissue Homogenate
Serpin Family C Member 1 (SERPINC1) ELISA KitSERPINC1 适用: 大鼠 Colorimetric Sandwich ELISA 12.5 ng/mL - 400 ng/mL Plasma, Serum, Urine
Serpin Family C Member 1 (SERPINC1) ELISA KitSERPINC1 适用: 小鼠 Colorimetric Sandwich ELISA 0.94 ng/mL - 60 ng/mL Plasma, Serum
Serpin Family C Member 1 (SERPINC1) ELISA KitSERPINC1 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Serpin Family C Member 1 (SERPINC1) ELISA KitSERPINC1 适用: 小鼠 Colorimetric Sandwich ELISA 0.937 ng/mL - 60 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Serpin Family C Member 1 (SERPINC1) ELISA KitSERPINC1 适用: 小鼠 Colorimetric Sandwich ELISA 3.125 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Serpin Family C Member 1 (SERPINC1) ELISA KitSERPINC1 适用: 小鼠 Colorimetric Sandwich ELISA 3.125-100 ng/mL Cell Culture Cells, Plasma, Serum, Urine
-
-
应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
-
样本量
- 100 μL
-
板类型
- Pre-coated
-
实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
-
实验精密度
-
Intra-Assay Precision (CV%): 4.4%, 7.8%, 4.2%
Inter-Assay Precision (CV%): 5.3%, 8.5%, 5.9% -
限制
- 仅限研究用
-
-
-
注意事项
- Avoid multiple freeze-thaw cycles.
-
储存条件
- -20 °C,4 °C
-
储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
-
有效期
- 12 months
-
-
- SERPINC1 (Serpin Family C Member 1 (SERPINC1))
-
别名
- SERPINC1
-
背景
-
Background: Antithrombin III is the most important inhibitor of thrombin and other coagulation proteinases. It belongs to the serine proteinase inhibitor (serpin) superfamily of inhibitors and structurally related proteins, which contain reactive centers that have evolved to attract and entrap certain proteinases. Inherited antithrombin III deficiency (AT3D) is a risk factor for the early development of venous thromboembolism (THPH7). Antithrombin III regulates clot formation both by inhibiting thrombin activity ly and by interfering with earlier stages of the clotting cascade. By in situ hybridization and quantitative analysis of DNA dosage in carriers of chromosome 1 deletions, AT3 is mapped to 1q23-q25. The AT3 gene has 7 exons. It contains 9 complete and 1 partial repetitive ALU sequence elements, which occur in the introns of the gene at a higher frequency (about 22 % of the intron sequence) than in the genome as a whole (about 5 %).
Gene Full Name: serpin family C member 1
-
基因ID
- 462
-
UniProt
- P01008
抗原 See all SERPINC1 ELISA试剂盒
-
