BIRC7 ELISA 试剂盒
Quick Overview for BIRC7 ELISA 试剂盒 (ABIN6720313)
抗原
See all BIRC7 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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Supplier Product No.
- koa0812
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Supplier
- Rockland
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原理
- Human Livin Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Production: Natural and recombinant human Livin. There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 20 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Baculoviral IAP Repeat-Containing 7 (BIRC7) ELISA Kit
BIRC7 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Baculoviral IAP Repeat-Containing 7 (BIRC7) ELISA KitBIRC7 适用: 人, 小鼠 AA 1-298 Colorimetric Sandwich ELISA 0.78-50 ng/mL Cell Culture Supernatant, Cell Lysate, Serum, Tissue Homogenate
Baculoviral IAP Repeat-Containing 7 (BIRC7) ELISA KitBIRC7 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
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应用备注
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human Livin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, cell lysates or tissue homogenates to each empty well. It is recommended that each human Livin standard solution and each sample be measured in duplicate.
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说明
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Standard: Expression system for standard: E.coli, Immunogen sequence: M1-S298
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样本量
- 100 μL
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- BIRC7 (Baculoviral IAP Repeat-Containing 7 (BIRC7))
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别名
- Livin
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背景
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Synonyms: Baculoviral IAP repeat containing 7, Baculoviral IAP repeat containing protein 7, Baculoviral IAP repeat-containing protein 7, BIRC 7, BIRC7, BIRC7_HUMAN, KIAP, Kidney inhibitor of apoptosis protein, LIVIN, Livin inhibitor of apoptosis, Melanoma inhibitor of apoptosis protein, ML IAP, ML-IAP, MLIAP, RING finger protein 50, RNF 50, RNF50
Background: BIRC7, also called KIAP or Livin, is a protein that in humans is encoded by the BIRC7 gene. This gene is mapped to 20q13.33. It encodes a member of the inhibitor of apoptosis protein (IAP) family, and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Elevated levels of the encoded protein may be associated with cancer progression and play a role in chemotherapy sensitivity. BIRC7 blocked apoptosis induced by BAX induction of cytochrome C release from mitochondria, and it also can block apoptosis induced by chemotherapeutic agents.
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基因ID
- 79444
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NCBI登录号
- NP_071444
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UniProt
- Q96CA5
抗原 See all BIRC7 ELISA试剂盒
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