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BIRC7 ELISA 试剂盒

BIRC7 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN625464
发货至: 中国
  • 抗原 See all BIRC7 ELISA试剂盒
    BIRC7 (Baculoviral IAP Repeat-Containing 7 (BIRC7))
    适用
    • 5
    • 3
    • 2
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human Livin ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
    样品类型
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes Human Livin.
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • 抗原 See all BIRC7 ELISA试剂盒
    BIRC7 (Baculoviral IAP Repeat-Containing 7 (BIRC7))
    别名
    Livin (BIRC7 产品)
    别名
    KIAP ELISA Kit, LIVIN ELISA Kit, ML-IAP ELISA Kit, MLIAP ELISA Kit, RNF50 ELISA Kit, EIAP/XLX ELISA Kit, XLX ELISA Kit, kiap ELISA Kit, livin ELISA Kit, ml-iap ELISA Kit, mliap ELISA Kit, rnf50 ELISA Kit, xEIAP ELISA Kit, RGD1562883 ELISA Kit, zgc:165605 ELISA Kit, E130019N06 ELISA Kit, Livin ELISA Kit, birc7 ELISA Kit, bird7a ELISA Kit, xEIAP/XLX ELISA Kit, xlx ELISA Kit, EIAP/XLX-B ELISA Kit, birc7b ELISA Kit, baculoviral IAP repeat containing 7 ELISA Kit, baculoviral IAP repeat-containing 7 ELISA Kit, baculoviral IAP repeat-containing 7 (livin) ELISA Kit, baculoviral IAP repeat containing 7 S homeolog ELISA Kit, baculoviral IAP repeat containing 7 L homeolog ELISA Kit, BIRC7 ELISA Kit, birc7 ELISA Kit, Birc7 ELISA Kit, birc7.S ELISA Kit, birc7.L ELISA Kit
    基因ID
    79444
    UniProt
    Q96CA5
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