MMP12 ELISA 试剂盒
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Quick Overview for MMP12 ELISA 试剂盒 (ABIN6720310)
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检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 62.5 pg/mL
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Supplier Product No.
- koa0622
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Supplier
- Rockland
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原理
- Mouse MMP-12 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: A18-C462 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) ELISA Kit
MMP12 适用: 大鼠 Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) ELISA KitMMP12 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Homogenates, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) ELISA KitMMP12 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) ELISA KitMMP12 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) ELISA KitMMP12 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) ELISA KitMMP12 适用: 人 AA 17-470 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) ELISA KitMMP12 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) ELISA KitMMP12 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) ELISA KitMMP12 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) ELISA KitMMP12 适用: 小鼠 AA 18-462 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
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说明
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Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse MMP-12 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin) to each empty well. It is recommended that each mouse MMP-12 standard solution and each sample be measured in duplicate.
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实验精密度
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Intra-Assay Precision (CV%): 7.3%, 7.4%, 7%
Inter-Assay Precision (CV%): 8.3%, 8.4%, 7.1% -
限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- MMP12 (Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12))
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别名
- MMP-12
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背景
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Background: Matrix metalloproteinase-12 (MMP12), also known as MME or ME, is an enzyme that in humans is encoded by the MMP12 gene. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.2. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. This gene may involved in tissue injury and remodeling.
Gene Full Name: matrix metallopeptidase 12
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基因ID
- 17381
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NCBI登录号
- NP_001307005
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UniProt
- P34960
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