MMP12 ELISA 试剂盒
(3 references)-
- 抗原 See all MMP12 ELISA试剂盒
- MMP12 (Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12))
- 抗原表位
- AA 18-462
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 62.5-4000 pg/mL
- 最低检测浓度
- 62.5 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse MMP-12
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: A18-C462 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: NSO
Immunogen sequence: A18-C462 - Featured
- Discover our best selling MMP12 ELISA Kit
- Top Product
- Discover our top product MMP12 ELISA Kit
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Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) ELISA Kit
MMP12 适用: 大鼠 Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) ELISA KitMMP12 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) ELISA KitMMP12 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) ELISA KitMMP12 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) ELISA KitMMP12 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Homogenates, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) ELISA KitMMP12 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12) ELISA KitMMP12 适用: 人 AA 17-470 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
SensoLyte® 520 MMP-12 Assay KitFRET Fluorometric
SensoLyte® 490 MMP-12 Assay KitFRET Fluorometric
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the peptidase M10A family.
- 板类型
- Pre-coated
- 实验流程
- mouse MMP-12 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for MMP-12 has been precoated onto 96-well plates. Standards(NSO, A18-C462) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse MMP-12 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse MMP-12 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse MMP-12 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 337, Standard deviation: 18.2, CV(%): 5.4
- Sample 2: n=16, Mean(pg/ml): 1472, Standard deviation: 92.74, CV(%): 6.3
- Sample 3: n=16, Mean(pg/ml): 2522, Standard deviation: 121.1, CV(%): 4.8,
- Sample 1: n=24, Mean(pg/ml): 426, Standard deviation: 27.69, CV(%): 6.5
- Sample 2: n=24, Mean(pg/ml): 1538, Standard deviation: 109.2, CV(%): 7.1
- Sample 3: n=24, Mean(pg/ml): 2768, Standard deviation: 157.8, CV(%): 5.7
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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: "Acute exacerbations of COPD are associated with significant activation of matrix metalloproteinase 9 irrespectively of airway obstruction, emphysema and infection." in: Respiratory research, Vol. 16, pp. 78, (2016) (PubMed).
: "Pulmonary C Fibers Modulate MMP-12 Production via PAR2 and Are Involved in the Long-Term Airway Inflammation and Airway Hyperresponsiveness Induced by Respiratory Syncytial Virus Infection." in: Journal of virology, Vol. 90, Issue 5, pp. 2536-43, (2016) (PubMed).
: "Myeloid-specific Fos-related antigen-1 regulates cigarette smoke-induced lung inflammation, not emphysema, in mice." in: American journal of respiratory cell and molecular biology, Vol. 53, Issue 1, pp. 125-34, (2015) (PubMed).
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: "Acute exacerbations of COPD are associated with significant activation of matrix metalloproteinase 9 irrespectively of airway obstruction, emphysema and infection." in: Respiratory research, Vol. 16, pp. 78, (2016) (PubMed).
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- 抗原 See all MMP12 ELISA试剂盒
- MMP12 (Matrix Metallopeptidase 12 (Macrophage Elastase) (MMP12))
- 别名
- MMP12 (MMP12 产品)
- 别名
- MMP12 ELISA Kit, AV378681 ELISA Kit, Mmel ELISA Kit, Mme ELISA Kit, HME ELISA Kit, ME ELISA Kit, MME ELISA Kit, MMP-12 ELISA Kit, matrix metallopeptidase 12 ELISA Kit, MMP12 ELISA Kit, Mmp12 ELISA Kit
- 背景
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Protein Function: May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3 (By similarity). .
Background: Matrix metalloproteinase-12(MMP12), also known as MME or ME, is an enzyme that in humans is encoded by the MMP12 gene. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.2. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. This gene may involved in tissue injury and remodeling.
Synonyms: Macrophage metalloelastase,MME,3.4.24.65,Matrix metalloproteinase-12,MMP-12,Mmp12,Mme, Mmel,
Full Gene Name: Macrophage metalloelastase
Cellular Localisation: Secreted, extracellular space, extracellular matrix. - 基因ID
- 17381
- UniProt
- P34960
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