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Angiotensin I Converting Enzyme 1 ELISA 试剂盒

This 小鼠 Angiotensin I Converting Enzyme 1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 Angiotensin I Converting Enzyme 1 in Cell Culture Supernatant, Plasma (heparin) 和 Serum.
Rockland
产品编号 ABIN6720307
Supplier Product No.: koa0338
发货至: 中国

Quick Overview for Angiotensin I Converting Enzyme 1 ELISA 试剂盒 (ABIN6720307)

抗原

See all Angiotensin I Converting Enzyme 1 (ACE) ELISA试剂盒
Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))

适用

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小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

93.8 pg/mL - 6000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (heparin), Serum
  • 最低检测浓度

    93.8 pg/mL

    Supplier Product No.

    koa0338

    Supplier

    Rockland

    原理

    Mouse ACE Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant mouse ACE. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 419pg/mL, 187.5pg/mL, 93.8pg/mL mouse ACE standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin) to each empty well. We recommend that each mouse ACE standard solution and each sample is measured in duplicate.

    说明

    Standard: Expression system for standard: NSO, Immunogen sequence: L35-Q1264

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all Angiotensin I Converting Enzyme 1 (ACE) ELISA试剂盒

    Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))

    别名

    ACE

    背景

    Synonyms: Angiotensin converting enzyme, CD143, DCP, Dcp1, Dipeptidyl carboxypeptidase I, Kininase II

    Background: Angiotensin-converting enzyme(ACE), an exopeptidase, is a circulating enzyme that participates in the body's renin-angiotensin system(RAS), which mediates extracellular volume(i.e. that of the blood plasma, lymph and interstitial fluid), and arterial vasoconstriction. It is secreted by pulmonary and renal endothelial cells and catalyzes the conversion of decapeptide angiotensin I to octapeptide angiotensin II. Using a DNA marker at the growth hormone gene locus, which they characterized as 'extremely polymorphic' and which showed no recombination with ACE, ACE was mapped to 17q22-q24, consistent with the in situ hybridization mapping to 17q23. ACE, or kininase II, is a dipeptidyl carboxypeptidase that plays an important role in blood pressure regulation and electrolyte balance by hydrolyzing angiotensin I into angiotensin II, a potent vasopressor, and aldosterone-stimulating peptide. The enzyme is also able to inactivate bradykinin, a potent vasodilator.

    基因ID

    11421

    NCBI登录号

    NP_033728

    UniProt

    P09470

    途径

    ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration
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