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IGFBP6 ELISA 试剂盒

This 人 IGFBP6 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 IGFBP6.
Rockland
产品编号 ABIN6720227
Supplier Product No.: koa0592
发货至: 中国

Quick Overview for IGFBP6 ELISA 试剂盒 (ABIN6720227)

抗原

See all IGFBP6 ELISA试剂盒
IGFBP6 (Insulin-Like Growth Factor Binding Protein 6 (IGFBP6))

适用

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检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

62.5 pg/mL - 4000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • 最低检测浓度

    62.5 pg/mL

    Supplier Product No.

    koa0592

    Supplier

    Rockland

    原理

    Human IGFBP6 Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant human IGFBP6. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human IGFBP6 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human IGFBP6 standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: NSO, Immunogen sequence: A25-G240

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all IGFBP6 ELISA试剂盒

    IGFBP6 (Insulin-Like Growth Factor Binding Protein 6 (IGFBP6))

    别名

    IGFBP6

    背景

    Synonyms: IBP 6, IBP-6, IBP6, IBP6_HUMAN, IGF binding protein 6, IGF-binding protein 6, IGFBP 6, IGFBP-6, IGFBP6, Insulin like growth factor binding protein 6, Insulin-like growth factor-binding protein 6

    Background: Insulin-like growth factor-binding protein 6(IGFBP6) is a protein that in humans is encoded by the IGFBP6 gene. The IGFBP6 gene is mapped to chromosome 12. In all, it has contained 216 amino acid protein with a calculated molecular mass of 22,847, The human keratinocyte cell line HaCat secretes IGFBP6 as an autocrine growth inhibitor. Recombinant IGFBP6 was also shown to inhibit growth of HaCat cells and other keratinocyte cell lines.

    基因ID

    3489

    NCBI登录号

    NP_002169

    UniProt

    P24592

    途径

    WNT signaling, Myometrial Relaxation and Contraction
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