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IGFBP2 ELISA 试剂盒

This 人 IGFBP2 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 IGFBP2.
Rockland
产品编号 ABIN6720226
Supplier Product No.: koa0201
发货至: 中国

Quick Overview for IGFBP2 ELISA 试剂盒 (ABIN6720226)

抗原

See all IGFBP2 ELISA试剂盒
IGFBP2 (Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2))

适用

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检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

62.5 pg/mL - 4000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • 最低检测浓度

    62.5 pg/mL

    Supplier Product No.

    koa0201

    Supplier

    Rockland

    原理

    Human IGFBP2 Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant human IGFBP2. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human IGFBP2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin or EDTA) or urine to each empty well. It is recommended that each human IGFBP2 standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: NSO, Immunogen sequence: E40-Q328

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all IGFBP2 ELISA试剂盒

    IGFBP2 (Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2))

    别名

    IGFBP2

    背景

    Synonyms: BP 2, BP2, IBP 2, IBP-2, IBP2, IBP2_HUMAN, IGF binding protein 2, IGF BP53, IGF-binding protein 2, IGF-BP53, IGFBP-2, IGFBP2, IGFBP53, Insulin Like Growth Factor Binding Protein 2, Insulin like growth factor binding protein 2 36 kDa, Insulin like growth factor-binding protein 2 precursor, Insulin-like growth factor-binding protein 2

    Background: Insulin-like growth factor-binding protein 2(IGFBP2), also called IBP2, is a protein that in humans is encoded by the IGFBP2 gene. It is mapped to 2q35. By structural analysis, it showed that the IGFBP2 gene consists of 4 exons with 3 introns of lengths 27.0, 1.0, and 1.9 kb. IGFBP2 secreted by metastatic cells recruits endothelia by modulating IGF1 mediated activation of the IGF type-I receptor on endothelial cells. The IGFBP2/IGF1/IGF1R and GAS6/MERTK signaling pathways are regulators of cancer-mediated endothelial recruitment.

    基因ID

    3485

    NCBI登录号

    NP_000588

    UniProt

    P18065

    途径

    Myometrial Relaxation and Contraction, Growth Factor Binding, Activated T Cell Proliferation
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