IGFBP2 ELISA 试剂盒
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Quick Overview for IGFBP2 ELISA 试剂盒 (ABIN6720032)
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See all IGFBP2 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 125 pg/mL
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Supplier Product No.
- koa0202
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Supplier
- Rockland
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原理
- Mouse IGFBP2 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: E35-Q305 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2) ELISA Kit
IGFBP2 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate, Urine
Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2) ELISA KitIGFBP2 适用: 小鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2) ELISA KitIGFBP2 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2) ELISA KitIGFBP2 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2) ELISA KitIGFBP2 适用: Pig Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2) ELISA KitIGFBP2 适用: Cow Colorimetric Sandwich ELISA 0.94 ng/mL - 60 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2) ELISA KitIGFBP2 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2) ELISA KitIGFBP2 适用: 大鼠 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2) ELISA KitIGFBP2 适用: Pig Colorimetric Sandwich ELISA 0.46 ng/mL - 30 ng/mL Plasma, Serum
Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2) ELISA KitIGFBP2 适用: 人 AA 40-328 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine
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说明
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Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL mouse IGFBP2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse IGFBP2 standard solution and each sample be measured in duplicate.
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实验精密度
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Intra-Assay Precision (CV%): 4.7%, 7.9%, 6.2%
Inter-Assay Precision (CV%): 5.3%, 8.9%, 7.4% -
限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- IGFBP2 (Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2))
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别名
- IGFBP2
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背景
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Background: Insulin-like growth factor-binding protein 2 (IGFBP2), also called IBP2, is a protein that in humans is encoded by the IGFBP2 gene. It is mapped to 2q35. By structural analysis, it showed that the IGFBP2 gene consists of 4 exons with 3 introns of lengths 27.0, 1.0, and 1.9 kb. IGFBP2 secreted by metastatic cells recruits endothelia by modulating IGF1 mediated activation of the IGF type-I receptor on endothelial cells. The IGFBP2/IGF1/IGF1R and GAS6/MERTK signaling pathways are regulators of cancer-mediated endothelial recruitment.
Gene Full Name: insulin-like growth factor binding protein 2
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基因ID
- 16008
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NCBI登录号
- NP_032368
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UniProt
- P47877
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途径
- Myometrial Relaxation and Contraction, Growth Factor Binding, Activated T Cell Proliferation
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