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CXCL14 ELISA 试剂盒

This 人 CXCL14 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 CXCL14 in Cell Culture Supernatant, Plasma (EDTA - heparin) 和 Serum.
Rockland
产品编号 ABIN6720223
Supplier Product No.: koa0761
发货至: 中国

Quick Overview for CXCL14 ELISA 试剂盒 (ABIN6720223)

抗原

See all CXCL14 ELISA试剂盒
CXCL14 (Chemokine (C-X-C Motif) Ligand 14 (CXCL14))

适用

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检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

125 pg/mL - 4000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • 最低检测浓度

    125 pg/mL

    Supplier Product No.

    koa0761

    Supplier

    Rockland

    原理

    Human CXCL14 Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant human CXCL14. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    ELISA_Dilution: 62.5pg/mL-4000pg/mL

    Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human CXCL14 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CXCL14 standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: E.coli, Immunogen sequence: S35-E111

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all CXCL14 ELISA试剂盒

    CXCL14 (Chemokine (C-X-C Motif) Ligand 14 (CXCL14))

    别名

    CXCL14

    背景

    Synonyms: BMAC, bolekine, BRAK, C-X-C motif chemokine 14, Chaemokine CXC motif ligand 14, Chemokine BRAK, CXC chemokine in breast and kidney, CXL14_HUMAN, JSC, Kec, Kidney-expressed chemokine CXC, KS1, MGC10687, MGC124510, MGC90667, MIP 2 gamma, MIP-2G, MIP2G, MIP2gamma, NJAC, PRO273, PSEC0212, Scyb14, Small Inducible Cytokine B14, Small inducible cytokine subfamily B (Cys-X-Cys) member 14 (BRAK), Small Inducible Cytokine subfamily B member 14, Small-inducible cytokine B14

    Background: Chemokine(C-X-C motif) ligand 14(CXCL14) is a small cytokine belonging to the CXC chemokine family that is also known as BRAK(for breast and kidney-expressed chemokine). This gene is mapped to 5q31.1. CXCL14 is constitutively expressed at high levels in many normal tissues, it has been found that fibroblasts are the primary source of CXCL14 and that CXCL14 is involved in the homeostasis of monocyte-derived macrophages rather than in inflammation. It is also a potent chemoattractant and activator of dendritic cells, is implicated in homing of these cells, and can stimulate the migration of activated NK cells. CXCL14 also inhibits angiogenesis, possibly as a result of its ability to block endothelial cell chemotaxis. In addition to it, CXCL14 is a potent chemokine for immature DCs, with a chemoattractive activity comparable to bacterial fMLP and MIP1A.

    基因ID

    9547

    NCBI登录号

    NP_004878

    UniProt

    O95715

    途径

    Autophagy
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