MBL2 ELISA 试剂盒
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Quick Overview for MBL2 ELISA 试剂盒 (ABIN6720207)
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检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 312 pg/mL
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Supplier Product No.
- koa0476
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Supplier
- Rockland
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原理
- Human MBP-C - MBL2 Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: E21-I248 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2) ELISA Kit
MBL2 适用: 人 Colorimetric Sandwich ELISA 1.563-100 ng/mL Serum
Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2) ELISA KitMBL2 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2) ELISA KitMBL2 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2) ELISA KitMBL2 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2) ELISA KitMBL2 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2) ELISA KitMBL2 适用: 小鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2) ELISA KitMBL2 适用: 小鸡 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2) ELISA KitMBL2 适用: 人 AA 21-248 Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2) ELISA KitMBL2 适用: 小鼠 AA 19-244 Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2) ELISA KitMBL2 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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说明
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Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human MBP-C standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human MBP-C standard solution and each sample be measured in duplicate.
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实验精密度
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Intra-Assay Precision (CV%): 5.7%, 4.4%, 7.6%
Inter-Assay Precision (CV%): 7.5%, 5.3%, 8.4% -
限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- MBL2 (Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2))
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别名
- MBP-C/MBL2
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背景
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Background: MBL2, also called mannose-binding lectin (protein C) 2, soluble or Mannose-binding lectin (MBL) is a lectin that is instrumental in innate immunity. MBL2 is mapped to chromosome 10q11.2-q21. It belongs to the class of collectins in the C-type lectin superfamily, whose function appears to be pattern recognition in the first line of defense in the pre-immune host. MBL2 recognizes carbohydrate patterns, found on the surface of a large number of pathogenic micro-organisms, including bacteria, viruses, protozoa and fungi. Binding MBL2 to a micro-organism results in activation of the lectin pathway of the complement system. Another important function of MBL2 is that this molecule binds senescent and apoptotic cells and enhances engulfment of whole, intact apoptotic cells, as well as cell debris by phagocytes.
Gene Full Name: mannose binding lectin 2
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基因ID
- 4153
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NCBI登录号
- NP_000233
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UniProt
- P11226
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途径
- Complement System, Positive Regulation of Immune Effector Process
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