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FGF1 ELISA 试剂盒

This 小鼠 FGF1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 FGF1.
Rockland
产品编号 ABIN6720192
Supplier Product No.: koa0675
发货至: 中国

Quick Overview for FGF1 ELISA 试剂盒 (ABIN6720192)

抗原

See all FGF1 ELISA试剂盒
FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))

适用

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小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

31.2 pg/mL - 2000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • 最低检测浓度

    31.2 pg/mL

    Supplier Product No.

    koa0675

    Supplier

    Rockland

    原理

    Mouse FGF1 Sandwich ELISA Kit for Quantitative Detection

    品牌

    AccuSignal™

    Analytical Method

    Quantitative

    特异性

    Production: Natural and recombinant mouse FGF1. There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • 应用备注

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse FGF1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse FGF1 standard solution and each sample be measured in duplicate.

    说明

    Standard: Expression system for standard: E.coli, Immunogen sequence: F16-D155

    样本量

    100 μL

    板类型

    Pre-coated

    限制

    仅限研究用
  • 储存条件

    4 °C,-20 °C

    储存方法

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    有效期

    12 months
  • 抗原 See all FGF1 ELISA试剂盒

    FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))

    别名

    FGF1

    背景

    Synonyms: Acidic fibroblast growth factor, aFGF, ECGF, Endothelial cell growth factor, FGF-1, Fgf1, FGF1_HUMAN, FGFA, Fibroblast growth factor 1, HBGF-1, Heparin-binding growth factor 1

    Background: Heparin-binding growth factor 1 is a protein that in humans is encoded by the FGF1 gene. The protein encoded by this gene is a member of the fibroblast growth factor(FGF) family. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. The FGF1 gene was mapped to chromosome 5q31.3-q33.2 by in situ hybridization.

    基因ID

    14164

    NCBI登录号

    NP_034327

    UniProt

    P61148

    途径

    RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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